Analysis of the PS II proteins MSP and CP43

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The goals of this proposal were two-fold: (1) to analyze the impact of mutations in the psbO gene (coding for the Mn-stabilizing protein, MSP) on O{sub 2}-evolution; and (2) to analyze membrane assembly in cyanobacteria, especially the effect of iron deficiency on this process. The mutations in the psbO gene were performed in the transformable and photoheterotrophic cyanobacterium Synechocystis sp. PCC6803, whereas the research with membrane assembly and iron deficiency was performed in the transformable strain Synechococcus sp. PCC7942. Our work with the Synechocystis psbO gene demonstrated that the MSP protein is not an essential protein but serves a regulatory ... continued below

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4 p.

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Sherman, L.A. July 1, 1995.

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Description

The goals of this proposal were two-fold: (1) to analyze the impact of mutations in the psbO gene (coding for the Mn-stabilizing protein, MSP) on O{sub 2}-evolution; and (2) to analyze membrane assembly in cyanobacteria, especially the effect of iron deficiency on this process. The mutations in the psbO gene were performed in the transformable and photoheterotrophic cyanobacterium Synechocystis sp. PCC6803, whereas the research with membrane assembly and iron deficiency was performed in the transformable strain Synechococcus sp. PCC7942. Our work with the Synechocystis psbO gene demonstrated that the MSP protein is not an essential protein but serves a regulatory function. We produced a deletion mutant, which we call {triangle}psbO. The {triangle}psbO strain was still capable of photosynthetic growth and evolved O{sub 2} at rates of 1/3 to 1/2 that of the wild type. We have characterized this strain in some detail and have reported the results in two publications in Biochemistry. The second of the these two papers was published during this grant period and is enclosed.

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4 p.

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OSTI as DE95015156

Medium: P; Size: 4 p.

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  • Other Information: PBD: [1995]

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  • Other: DE95015156
  • Report No.: DOE/ER/14028--5
  • Grant Number: FG02-89ER14028
  • DOI: 10.2172/100110 | External Link
  • Office of Scientific & Technical Information Report Number: 100110
  • Archival Resource Key: ark:/67531/metadc625563

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  • July 1, 1995

Added to The UNT Digital Library

  • June 16, 2015, 7:43 a.m.

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  • April 13, 2017, 12:20 p.m.

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Sherman, L.A. Analysis of the PS II proteins MSP and CP43, report, July 1, 1995; United States. (digital.library.unt.edu/ark:/67531/metadc625563/: accessed October 23, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.