Rapid mass spectrometric DNA diagnostics for assessing microbial community activity during bioremediation. 1998 annual progress report

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'Biological activity has often been attributed to changes in pollutant profiles found in contaminated soils when abiotic processes actually caused pollutant removal. Careful evaluation of bioremediation necessitates that all transformation and removal pathways are anticipated so that the pathways are controlled or monitored. The author''s work is directed at evaluating a monitoring strategy that relies on the combined use of DNA diagnostic procedures and mass spectrometry as the detection scheme. The intent is to track bioremediation by measuring the occurrence of genes in soil samples that are known to code for enzymes capable of degrading specific pollutants. Matrix-assisted-laser-desorption- ionization time-of-flight ... continued below

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3 pages

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Benner, W.H. June 1, 1998.

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Description

'Biological activity has often been attributed to changes in pollutant profiles found in contaminated soils when abiotic processes actually caused pollutant removal. Careful evaluation of bioremediation necessitates that all transformation and removal pathways are anticipated so that the pathways are controlled or monitored. The author''s work is directed at evaluating a monitoring strategy that relies on the combined use of DNA diagnostic procedures and mass spectrometry as the detection scheme. The intent is to track bioremediation by measuring the occurrence of genes in soil samples that are known to code for enzymes capable of degrading specific pollutants. Matrix-assisted-laser-desorption- ionization time-of-flight mass spectrometry (MALDI-TOF-MS) offers the possibility for automation and high throughput PNA detection as is needed to track the course of bioremediation over large polluted areas.'

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3 pages

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  • Other: DE00013429
  • Report No.: EMSP-54698--98
  • Grant Number: NONE
  • DOI: 10.2172/13429 | External Link
  • Office of Scientific & Technical Information Report Number: 13429
  • Archival Resource Key: ark:/67531/metadc624317

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  • June 1, 1998

Added to The UNT Digital Library

  • June 16, 2015, 7:43 a.m.

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  • June 13, 2016, 6:16 p.m.

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Benner, W.H. Rapid mass spectrometric DNA diagnostics for assessing microbial community activity during bioremediation. 1998 annual progress report, report, June 1, 1998; California. (digital.library.unt.edu/ark:/67531/metadc624317/: accessed October 16, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.