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Dielectrophoretic manipulation of particles for use in microfluidic devices

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Amplification and hybridization of DNA are commonly used techniques to verify the presence of a specific DNA sequence in a test sample. Automatic sample handling to concentrate and purify sample prior to amplification is desirable both from the cost standpoint and from the standpoint of reducing the possibility of sample contamination. This paper explores the use of the dielectrophoretic force to manipulate DNA, Bacillus globigii spores, and Erwinia herbicola bacteria to provide concentration and purification as part of the sample handling functions in biological monitoring equipment. It was found that for what would be considered a typical microfabricated structure with ... continued below

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1.1 Megabytes pages

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Belgrader, P; Bettencourt, K; Hamilton, J; Miles, R & Nasarabadi, S June 23, 1999.

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This article is part of the collection entitled: Office of Scientific & Technical Information Technical Reports and was provided by UNT Libraries Government Documents Department to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 14 times . More information about this article can be viewed below.

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Description

Amplification and hybridization of DNA are commonly used techniques to verify the presence of a specific DNA sequence in a test sample. Automatic sample handling to concentrate and purify sample prior to amplification is desirable both from the cost standpoint and from the standpoint of reducing the possibility of sample contamination. This paper explores the use of the dielectrophoretic force to manipulate DNA, Bacillus globigii spores, and Erwinia herbicola bacteria to provide concentration and purification as part of the sample handling functions in biological monitoring equipment. It was found that for what would be considered a typical microfabricated structure with electrode gaps at 30 {micro}m operating at 5V, that concentration of the particles is very effective.

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1.1 Megabytes pages

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  • IMECE 299 Microfluidics Symposium, Nashville, TN (US), 11/14/1999--11/19/1999

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  • Report No.: UCRL-JC-133768
  • Report No.: YN0100000
  • Grant Number: W-7405-ENG-48
  • Office of Scientific & Technical Information Report Number: 14568
  • Archival Resource Key: ark:/67531/metadc623512

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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  • June 23, 1999

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  • June 16, 2015, 7:43 a.m.

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  • May 6, 2016, 2:22 p.m.

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Belgrader, P; Bettencourt, K; Hamilton, J; Miles, R & Nasarabadi, S. Dielectrophoretic manipulation of particles for use in microfluidic devices, article, June 23, 1999; California. (digital.library.unt.edu/ark:/67531/metadc623512/: accessed January 24, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.