Lymphocytes from wasted mice express enhanced spontaneous and {gamma}-ray-induced apoptosis

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Mice bearing the autosomal recessive mutation wasted (wst/wst) display a disease pattern including faulty repair of DNA damage in lymphocytes after radiation exposure, neurologic abnormalities, and immunodeficiency. Many of the features of this mouse model have suggested a premature or increased spontaneous frequency of apoptosis in thymocytes; past work has shown an inability to establish cultured T cell lines, an abnormally high death rate of stimulated T cells in culture, and an increased sensitivity of T cells to the killing effects of ionizing radiations in wst/wst mice relative to controls. The experiments reported here were designed to examine splenic and ... continued below

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31 p.

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Woloschak, G.E.; Chang-Liu, Chin-Mei; Chung, Jen & Libertin, C.R. September 1, 1993.

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Description

Mice bearing the autosomal recessive mutation wasted (wst/wst) display a disease pattern including faulty repair of DNA damage in lymphocytes after radiation exposure, neurologic abnormalities, and immunodeficiency. Many of the features of this mouse model have suggested a premature or increased spontaneous frequency of apoptosis in thymocytes; past work has shown an inability to establish cultured T cell lines, an abnormally high death rate of stimulated T cells in culture, and an increased sensitivity of T cells to the killing effects of ionizing radiations in wst/wst mice relative to controls. The experiments reported here were designed to examine splenic and thymic lymphocytes from wasted and control mice for signs of early apoptosis. Our results revealed enhanced expression of Rp-8 mRNA (associated with apoptosis) in thymic lymphocytes and reduced expression in splenic lymphocytes of wst/wst mice relative to controls; expression of Rp-2 and Td-30 mRNA (induced during apoptosis) were not detectable in spleen or thymus. Higher spontaneous DNA fragmentation was observed in wasted mice than in controls; however, {gamma}-ray-induced DNA fragmentation peaked at a lower dose and occurred to a greater extent in wasted mice relative to controls. These results provide evidence for high spontaneous and {gamma}-ray-induced apoptosis in T cells of wasted mice as a mechanism underlying the observed lymphocyte and DNA repair abnormalities.

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31 p.

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OSTI as DE93040889

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  • Other Information: PBD: [1993]

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  • Other: DE93040889
  • Report No.: ANL/BIM/PP--78894
  • Report No.: ANL/CMB/PP--80200
  • Grant Number: W-31109-ENG-38
  • DOI: 10.2172/139782 | External Link
  • Office of Scientific & Technical Information Report Number: 139782
  • Archival Resource Key: ark:/67531/metadc622746

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Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • September 1, 1993

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  • June 16, 2015, 7:43 a.m.

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  • Dec. 15, 2015, 6:12 p.m.

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Woloschak, G.E.; Chang-Liu, Chin-Mei; Chung, Jen & Libertin, C.R. Lymphocytes from wasted mice express enhanced spontaneous and {gamma}-ray-induced apoptosis, report, September 1, 1993; Illinois. (digital.library.unt.edu/ark:/67531/metadc622746/: accessed October 19, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.