Control of sugar transport and metabolism in Zymomonas mobilis. Final report

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Description

This research deals with the physiology and genetics of sugar transport and metabolic control in Zymomonas mobilis. The specific objectives of the grant as as follows: First, the complex interactions of transcriptional, post-transcriptional and translational control mechanisms on regulation of the glf operon will be investigated. Second, the structure and function of the unique glucose facilitator will be examined by a combination of in vitro and in vivo approaches, making use of the genetically reconstituted system in E. coli. Third, the possibility that physical association or indirect interactions between the glucose facilitator and glucokinase are involved in metabolic control will ... continued below

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15 p.

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Conway, T. September 1, 1995.

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This report is part of the collection entitled: Office of Scientific & Technical Information Technical Reports and was provided by UNT Libraries Government Documents Department to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 60 times . More information about this report can be viewed below.

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  • Conway, T. Ohio State Univ., Columbus, OH (United States)

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Description

This research deals with the physiology and genetics of sugar transport and metabolic control in Zymomonas mobilis. The specific objectives of the grant as as follows: First, the complex interactions of transcriptional, post-transcriptional and translational control mechanisms on regulation of the glf operon will be investigated. Second, the structure and function of the unique glucose facilitator will be examined by a combination of in vitro and in vivo approaches, making use of the genetically reconstituted system in E. coli. Third, the possibility that physical association or indirect interactions between the glucose facilitator and glucokinase are involved in metabolic control will be analyzed. Fourth, the Z. mobilis glucose transport and phosphorylation system will be utilized to metabolically engineer recombinant E. coli with altered cell pool metabolite profiles. Work on the third and fourth objectives is complete, work on the first and second objectives is progressing nicely. Publication of this work has been admittedly slow, due primarily to a change n location of the research program from the University of Nebraska to The Ohio State University. However, it should be noted that much of the unpublished data outlined below represented completed studies, and are contained in graduate student theses which are being prepared for submission this summer. Since a full year remains in the current funding period, and the new laboratory is now up and running, we fully expect to make reasonable progress on the remaining objectives and to publish the results in a timely fashion.

Physical Description

15 p.

Notes

OSTI as DE95017348

Medium: P; Size: 15 p.

Source

  • Other Information: PBD: [1995]

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  • Other: DE95017348
  • Report No.: DOE/ER/20002--T2
  • Grant Number: FG02-90ER20002
  • DOI: 10.2172/100131 | External Link
  • Office of Scientific & Technical Information Report Number: 100131
  • Archival Resource Key: ark:/67531/metadc621923

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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Creation Date

  • September 1, 1995

Added to The UNT Digital Library

  • June 16, 2015, 7:43 a.m.

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  • April 7, 2017, 2:50 p.m.

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Conway, T. Control of sugar transport and metabolism in Zymomonas mobilis. Final report, report, September 1, 1995; United States. (digital.library.unt.edu/ark:/67531/metadc621923/: accessed May 24, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.