Identification of methanococcus jannaschii proteins in 2-D gel electrophoresis patterns by mass spectrometry.

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The genome of Methanococcus jannaschii has been sequenced completely and has been found to contain approximately 1,770 predicted protein-coding regions. When these coding regions are expressed and how their expression is regulated, however, remain open questions. In this work, mass spectrometry was combined with two-dimensional gel electrophoresis to identify which proteins the genes produce under different growth conditions, and thus investigate the regulation of genes responsible for functions characteristic of this thermophilic representative of the methanogenic Archaea.

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4 p.

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Liang, X. June 10, 1998.

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The genome of Methanococcus jannaschii has been sequenced completely and has been found to contain approximately 1,770 predicted protein-coding regions. When these coding regions are expressed and how their expression is regulated, however, remain open questions. In this work, mass spectrometry was combined with two-dimensional gel electrophoresis to identify which proteins the genes produce under different growth conditions, and thus investigate the regulation of genes responsible for functions characteristic of this thermophilic representative of the methanogenic Archaea.

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4 p.

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OSTI as DE00010638

Medium: P; Size: 4 pages

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  • 46th American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics, Orlando, FL (US), 06/01/1998--06/05/1998

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  • Report No.: ANL/CMB/CP-95616
  • Grant Number: W-31109-ENG-38
  • Office of Scientific & Technical Information Report Number: 10638
  • Archival Resource Key: ark:/67531/metadc619915

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Office of Scientific & Technical Information Technical Reports

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  • June 10, 1998

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  • June 16, 2015, 7:43 a.m.

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  • April 10, 2017, 8:10 p.m.

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Liang, X. Identification of methanococcus jannaschii proteins in 2-D gel electrophoresis patterns by mass spectrometry., article, June 10, 1998; Illinois. (digital.library.unt.edu/ark:/67531/metadc619915/: accessed October 20, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.