Coelomic Fluid Protein Profile in Earthworms Following Bacterial Challenge.

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Proteomic techniques were used to evaluate the protein profile of the earthworm, (Lumbricus terrestris), following a bacterial challenge. One control group received no injection; a second control group received injections of phosphate buffer solution (PBS). The experimental group received injections of PBS containing (Aeromonas hydrophila). After incubation for 12 hours at 20°C, coelomic fluid was collected from each group for analysis by 2-D electrophoresis. There were significant differences in spot appearance and density between control and experimental groups. Sixteen spots showed a two-fold increase in density and 63 showed at least a two-fold decrease in density between samples from control ... continued below

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Brooks, Geoffrey Lance December 2006.

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  • Brooks, Geoffrey Lance

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Proteomic techniques were used to evaluate the protein profile of the earthworm, (Lumbricus terrestris), following a bacterial challenge. One control group received no injection; a second control group received injections of phosphate buffer solution (PBS). The experimental group received injections of PBS containing (Aeromonas hydrophila). After incubation for 12 hours at 20°C, coelomic fluid was collected from each group for analysis by 2-D electrophoresis. There were significant differences in spot appearance and density between control and experimental groups. Sixteen spots showed a two-fold increase in density and 63 showed at least a two-fold decrease in density between samples from control and bacteria-challenged earthworms, respectively, suggesting up- and down-modulation of proteins potentially involved in the earthworm's response to bacterial challenge.

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  • December 2006

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  • May 5, 2008, 3:01 p.m.

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  • July 1, 2015, 12:38 p.m.

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Brooks, Geoffrey Lance. Coelomic Fluid Protein Profile in Earthworms Following Bacterial Challenge., thesis, December 2006; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc5476/: accessed September 20, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .