Experiment Station Record, Volume 92, January-June, 1945 Page: 11
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1945] AGRICULTURAL AND BIOLOGICAL CHEMISTRY 11
regarded in view of the probable error of the estimation. It is indicated, however,
that a control assay with pure ascorbic acid should be carried out for any new stock
of animals. Factors that might influence the assay, such as fasting, cod-liver oil
feeding, age, and re-use of animals, are discussed. It was found possible to assay
by this method substances of known and questionable potency as determined by other
methods, such as dehydroascorbic acid, d-glucoascorbic acid, d-isoascorbic acid,
2-ketogulonic acid, and iron ascorbate. A study of the relative effectiveness of oral
and parenteral administration of ascorbic acid suggested, a loss of 27 percent when
the vitamin was fed by mouth.
Factors influencing the bioassay of vitamin E, B. R. HOMRICH (Jour. Nutr.,
26 (1943), No. 4, pp. 391-398).-An investigation of some of the factors influencing
the bio-assay of vitamin E following methods essentially as described by Palmer
(E. S. R., 79, p. 716) is reported with suggestions for certain improvements in
technic. These include the discarding of rats having less than 7 and more than 14
implantations, dosing during the early days of pregnancy rather than prior to mating,
autopsying prior to parturition to eliminate possible errors due to infanticide,
and using a fat-free basal diet during the dosing period because of the destructive
effect on vitamin E activity of lard and cod-liver oil.
[Studies of Penicillium and penicillin] (Jour. Bact., 48 (1944), No. 1, pp. 120'122).-Abstracts
of the following papers are included: The Biological Whereabout
of the Penicillia, by C. Thom (pp. 120-121); Biological Methods of Penicillin
Assay, by R. B. McCormack, H. P. Jones, and G. Plaut, Microbiological Methods
of Identification of Penicillin, by G. Rake and D. M. Hamre, and Development in
Vivo and in Vitro of Strains of Organisms Resistant to Penicillin, by C. M. McKee
and W. B. Dunham (all p. 121); and Penicillin and Therapy, by C. F. Church
A method for the assay of penicillin, N. G. HEATLEY (B i o c h em. Jour., 33
(1944), No. 1, pp. 61-65, illus. 1).-Full practical details are given of a method of
assay applicable also to certain other antibiotics; it is said to compare' favorably as
regards speed and accuracy with most other technics and has been in routine use
for nearly 4 yr. Less than 1 cc. of solution (which need not be sterile) is required
for an assay, and the presence of ether or chloroform does not interfere. Quantitative
information on limits of accuracy is given..
Disinfectant testing-F. D. A. method: Official method of U. S. Food and
Drug Administration, U. S. Department of Agriculture, and National Association
of Insecticide and Disinfectant Manufacturers for determination of phenol
coefficients of disinfectants, G. L. A. RUEHLE and C. M. BREWER (Blue Book
[Soap and Sanit. Chem.], 1944, pp. 205, 207-212).
Disinfectants and antiseptics: A summary of scientific advances reported during
1943, E. G. KLARMANN (Soap and Sanit. Chem., 20 (1944), No. 1, pp. 109117,
Methods for estimating the moisture content of foods in the dehydrator and
in the equalizing bins, G. J. BOUYoucos and R . E. MARSHALL (Michigan Sta.
Tech. But. 197 (1944), pp. 29, illus. 9).-This bulletin presents working details illustrations
of the equipment, and further experimental results for the method described
in a preliminary report (E. S. R., 91, p. 119) for estimating the moisture
content of foods in the dehydrator. In addition, the method is considered in detail
in its application to determining moisture in material undergoing equalizing and
final drying in bins. In this case the determination is accomplished by measuring
the temperature of the warmed incoming air and that of the air after it has passed'
the product and is being exhausted from the bin. While moisture is being evaporated
from the material the temperature of the exhaust air is considerably below
that entering the bin. When the product reaches a low moisture content the amount
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U.S. Department of Agriculture. Agricultural Research Administration. Office of Experiment Stations. Experiment Station Record, Volume 92, January-June, 1945, book, 1947; Washington D.C.. (digital.library.unt.edu/ark:/67531/metadc5064/m1/24/: accessed March 24, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.