Experiment Station Record, Volume 92, January-June, 1945 Page: 4
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4 EXPERIMENT STATION RECORD [Vol. 92
Structure and Gene Action, by A. Gulick (pp. 1-39) (Univ. Mo.); Specificity,
Classification, and Mechanism of Action of the Glycosidases, by W. W. Pigman
(pp. 41-74); The Transamination Reaction, by R. M. Herbst (pp. 75-97); Tyrosinase,
by J. M. Nelson and C. R. Dawson (pp. 99-152); Gramicidin, Tyrocidine,
and Tyrothricin, by R. D. Hotchkiss (pp. 153-199); Biological Energy Transformation
and the Cancer Problem, by V. R. Potter (pp. 201-256); The Influence of
I-ormones on Enzymatic Reactions, by H. Jensen and L. E. Tenenbaum (pp. 257267);
and The Absorption Spectra of Vitamins, Hormones, and Enzymes, by W. R.
Brode (pp. 269-311) (Ohio State Univ.). Bibliographies terminate the individual
sections. Author and subject indexes of this volume and a cumulative index of
volumes I-IV are included.
On the oxidative decomposition of hexosediphosphate by barley: The role of
ascorbic acid, W. O. JAMES, C. R. C. HEARD, and G. M. JAMES (New Phytol.,
43 (1944), No. 1, pp. 62-74, illus. 3). "Barley saps incubated for 24 hr. at 30 C.
with hexosediphosphate were analyzed for phosphate fractions. In the presence
of thymol and NaF they showed a loss of hexosediphosphate and a rise in the ratio
of unhydrolyzable/3 hr.-hydrolyzable phosphate. Reasons are given for taking
the unhydrolyzable ester formed to be phosphoglycerate. This effect was increased
by addition of ascorbic acid, but the presence of M/2,200 CuSO, abolished
its action. In the presence of' iodoacetate or NaF the digests accumulated alkalilabile
phosphate esters (triosephosphates). Cyanide and bisulfite increased this
accumulation and phosphoglycerate was not formed. In manometric experiments,
clarified saps with hexosediphosphate absorbed little or no 02. On addition of
ascorbic acid, rapid 02 uptake occurred in excess of the oxygen uptake caused by
ascorbic acid alone. Addition of coenzyme I to sap with ascorbic acid caused a
large increase of 02 uptake, far beyond the O0 equivalent of the coenzyme added.
Hexosediphosphate still further increased this 02 uptake. The linkage between the
oxidation and glycolysis stages of respiration suggested by these results is elaborated."
Amino acid nutrition of Lactobacillus arabinosus, S. SHANKMAN (Jour. Biol.
Chem., 150 (1943), No. 2, pp. 305-310, illus. 2).--Preliminary experiments were carried
out on a synthetic medium composed of 14 amino acids, salts, dextrose, sodium
acetate, vitamins, and adenine, guanine, and uracil, with the exception that a different
amino acid was omitted in each run. Lack of growth of the organism as
shown by turbidity and acid production was taken as evidence of the essential
character of the amino acid. As a result of these tests, cystine, methionine, tryptophan,
leucine, isoleucine, valine, glutamic acid, and threonine were shown to be
essential nutrilities for L. arabinosus. Subsequent experiments conducted to determine
the level at which the amino acid promoted growth led to the establishment
of quantitative requirements. Arginine was' shown to have two levels of stimulation,
with an intermediate range of inhibition.
The analysis of eight amino acids by a microbiological method, S. SHANKMAN,
M. S. DUNN, and L. B. RUBIN. (Univ. Calif. et al.). (four. Biol. Chem., 150
(1943), No. 2, pp. 477-478).-Use of the medium developed in the above study
/of the nutritive requirements of Lactobacillus arabinosus permitted quantitative
determination by standard biological technics of each of the eight amino acids
shown by the above tests to be essential nutrilites for the test organism. The experimental
data and the results of the analyses reported indicate that the average
deviation of the quantities of amino acids found differed from that of the amino
acids present by 3.2 percent. It is concluded, therefore, that the accuracy of this
analysis compares favorably with that of other microbiological assays.
The microbiological analysis of seven amino acids with Lactobacillus casei,
S. SHANKMAN, M. S. DUNN, and L. B. RUBIN. (Univ. Calif.). (four. Biol.
Chem., 151 (1943), No. 2, pp. 511-514).-The method described for the micro
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U.S. Department of Agriculture. Agricultural Research Administration. Office of Experiment Stations. Experiment Station Record, Volume 92, January-June, 1945, book, 1947; Washington D.C.. (digital.library.unt.edu/ark:/67531/metadc5064/m1/17/: accessed March 23, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.