Studies on Lipoprotein Specificity of Human Plasma Lecithin Cholesterol Acyltransferase

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Huian plasma high-density lipoprotein (HDL) were isolated by a procedure employing polyanion precipitation and column chromatography. Lipid and protein composition of the HDL isolated by this method was found to be similar to another HDL preparation isolated by ultracentrifugation. However, minor differences were noted, including a higher phospholipid and apoproteinE content and lower triglyceride content of the HDL isolated by column chromatography. Four subfraction of HDL were obtained following chromatography on an anion exchange column. The subfraction four had the highest esterified to free cholesterol ratio, the second highest phospholipid to unesterified cholesterol, and the lowest molecular weight. In addition ... continued below

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i, 65 leaves: ill.

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Jahani, Mehrnoosh May 1981.

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  • Jahani, Mehrnoosh

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Huian plasma high-density lipoprotein (HDL) were isolated by a procedure employing polyanion precipitation and column chromatography. Lipid and protein composition of the HDL isolated by this method was found to be similar to another HDL preparation isolated by ultracentrifugation. However, minor differences were noted, including a higher phospholipid and apoproteinE content and lower triglyceride content of the HDL isolated by column chromatography. Four subfraction of HDL were obtained following chromatography on an anion exchange column. The subfraction four had the highest esterified to free cholesterol ratio, the second highest phospholipid to unesterified cholesterol, and the lowest molecular weight. In addition it was consistently coincided with lecithin: cholesterol acyltransferase (LCAT) activity and found to be the best substrate for the enzyme.

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i, 65 leaves: ill.

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  • May 1981

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  • May 10, 2015, 6:16 a.m.

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  • Feb. 3, 2017, 1:50 p.m.

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Jahani, Mehrnoosh. Studies on Lipoprotein Specificity of Human Plasma Lecithin Cholesterol Acyltransferase, thesis, May 1981; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc504553/: accessed August 15, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .