Isolation and Partial Characterization of Pigment Mutants of Corynebacterium poinsettie ATCC 9682 Page: 2
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Wariso, Benjamin A., Isolation and Partial Characteriza-
tion of Piglent Mutants of Corynebacterium poinsettiae ATCC
9682. Master of Science (Biology) , August, 1980, 39 pp.,
4 tables, 5 figures, bibliography, 37 titles.
Carotenoid pigments were extracted from Corynebacteriuma
poinsettiae (wild type) ATCC 9682, and from 108 mutants ob-
tained by exposure of a streptomycin resistant strain of C.
poinsettiae to ultra-violet light irradiation and N-methyl-
N'-nitro-N-nitrosoguanidine. The pigments were characterized
by their absorption maxima, Rf-values, and partition ratios
in petroleum ether and methanol. Thin layer chromatography
was used to compare pigments of the wild type with those of
the mutants. Possible biosynthetic pathways in carotenoid
synthesis of the wild type were postulated on the basis of
the observed genetic blocks. Mutants were found which sug-
gested the existence of a linear pathway in carotenoid syn-
thesis from the aliphatic C40 molecule to the bi-cyclic
C50-diol. Other mutants suggested possible alternative
pathways in the biosynthesis of these pigments or the
presence of intermediates not detectable by thin layer
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Wariso, Benjamin A. Isolation and Partial Characterization of Pigment Mutants of Corynebacterium poinsettie ATCC 9682, thesis, August 1980; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc504530/m1/2/: accessed August 20, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .