Isolation and Characterization of Two Enzyme Proteins Catalyzing Oxido-Reduction at C-9 and C-15 of Prostaglandins from Swine Kidney

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Two swine kidney proteins (PI 4.8 and 5.8) both possessing 9-prostaglandin ketoreductase (9-PGKR) and 15-hydroxyprostaglandin dehydrogenase (15-PGDH) activities were purified to homogeneity. Purification increased specific activities in parallel. Molecular weight, subunit size, amino acid composition, coenzyme and substrate specificity and antigenicity of both proteins were similar. Gel filtration and SDS-polyacrylamide gel electrophoresis molecular weights of 29,500 and 29,000, respectively, suggested a single subunit. Although a variety of prostaglandins served as substrates, the best for 15-PGDH was PGB, while PGA_1-GSH showed the lowest Km for 9-PGKR. Rabbit antibody against the PI 5.8 protein crossreacted with both purified renal enzymes and with ... continued below

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v, 78 leaves : ill.

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Chang, David Guey-Bin December 1980.

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  • Chang, David Guey-Bin

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Two swine kidney proteins (PI 4.8 and 5.8) both possessing 9-prostaglandin ketoreductase (9-PGKR) and 15-hydroxyprostaglandin dehydrogenase (15-PGDH) activities were purified to homogeneity. Purification increased specific activities in parallel. Molecular weight, subunit size, amino acid composition, coenzyme and substrate specificity and antigenicity of both proteins were similar. Gel filtration and SDS-polyacrylamide gel electrophoresis molecular weights of 29,500 and 29,000, respectively, suggested a single subunit. Although a variety of prostaglandins served as substrates, the best for 15-PGDH was PGB, while PGA_1-GSH showed the lowest Km for 9-PGKR. Rabbit antibody against the PI 5.8 protein crossreacted with both purified renal enzymes and with extracts from rat spleen, lung, heart, aorta, and liver.

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v, 78 leaves : ill.

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  • December 1980

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  • May 10, 2015, 6:16 a.m.

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  • Feb. 23, 2017, 12:30 p.m.

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Chang, David Guey-Bin. Isolation and Characterization of Two Enzyme Proteins Catalyzing Oxido-Reduction at C-9 and C-15 of Prostaglandins from Swine Kidney, thesis, December 1980; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc504258/: accessed June 25, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .