An Assay Method for Determining Extra-Cellular Lipases from Pseudomonas aeruginosa

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The applicability of an isotopically labelled assay system to determine the lipase production in Pseudomonas aeruginosa was evaluated. Supernatant from cultures of Pseudomonas aeruginosa grown in a medium containing olive oil was incubated with a substrate containing labelled trioleate. Fatty acids were isolated by means of a liquid-liquid partition system. Enzyme activity was determined by measuring the amounts of free fatty acid by liquid scintillation counting. Findings indicate that the isotopicallylabelled, liquid-liquid partitioning assay is reliable, sensitive and adaptable to rapid assay conditions. It was also determined that different strains of Pseudomonas aeruginosa produce varying amounts of lipase. Partial purification ... continued below

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vi, 39 leaves : illustrations

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Christensen, John N. May 1978.

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  • Christensen, John N.

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The applicability of an isotopically labelled assay system to determine the lipase production in Pseudomonas aeruginosa was evaluated. Supernatant from cultures of Pseudomonas aeruginosa grown in a medium containing olive oil was incubated with a substrate containing labelled trioleate. Fatty acids were isolated by means of a liquid-liquid partition system. Enzyme activity was determined by measuring the amounts of free fatty acid by liquid scintillation counting. Findings indicate that the isotopicallylabelled, liquid-liquid partitioning assay is reliable, sensitive and adaptable to rapid assay conditions. It was also determined that different strains of Pseudomonas aeruginosa produce varying amounts of lipase. Partial purification of supernatant by gel filtration produced two protein peaks showing enzymatic activity.

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vi, 39 leaves : illustrations

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UNT Theses and Dissertations

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  • May 1978

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  • May 10, 2015, 6:16 a.m.

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  • Sept. 20, 2016, 11:07 a.m.

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Citations, Rights, Re-Use

Christensen, John N. An Assay Method for Determining Extra-Cellular Lipases from Pseudomonas aeruginosa, thesis, May 1978; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc504214/: accessed January 23, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .