Diphenyloxazole Metabolism by Aryl Hydrocarbon Hydroxylase

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2,5-Diphenyloxazole (PPO) was tested as a potential alternate inducer for the aryl hydrocarbon hydroxylase (AHH) system. Its apparent lack. of carcinogenicity and toxicity provide a possible system for investigation of enzyme systems related to chemical carcinogenesis without exposure of the researcher to potent carcinogenic compounds. These studies found PPO to be an inducer of AHH in cultured human lymphocytes. When PPO was utilized as a substrate for the AHH assay system, the major metabolites produced were strongly fluorescent. A simple fluorometric assay was developed which employed PPO as the substrate and which measured constitutive activity more efficiently than similar assays ... continued below

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vi, 71 leaves: ill.

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Abreu, Mary E. December 1976.

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  • Abreu, Mary E.

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2,5-Diphenyloxazole (PPO) was tested as a potential alternate inducer for the aryl hydrocarbon hydroxylase (AHH) system. Its apparent lack. of carcinogenicity and toxicity provide a possible system for investigation of enzyme systems related to chemical carcinogenesis without exposure of the researcher to potent carcinogenic compounds. These studies found PPO to be an inducer of AHH in cultured human lymphocytes. When PPO was utilized as a substrate for the AHH assay system, the major metabolites produced were strongly fluorescent. A simple fluorometric assay was developed which employed PPO as the substrate and which measured constitutive activity more efficiently than similar assays using benzo(a)pyrene as the substrate. Quantitation of both basal and induced lymphocyte AHH metabolism of PPO may be applicable to human population studies and may provide a tool to determine possible genetic variables with respect to carcinogen metabolism related to cancer risk.

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vi, 71 leaves: ill.

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  • December 1976

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  • May 10, 2015, 6:16 a.m.

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  • June 27, 2016, 1:52 p.m.

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Abreu, Mary E. Diphenyloxazole Metabolism by Aryl Hydrocarbon Hydroxylase, thesis, December 1976; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc503973/: accessed October 19, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .