Studies on the Purification and Phosphorylation of Phosphofructokinase from Ascaris suum

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A new procedure has been developed to concentrate the phosphofructokinase from muscle of Ascaris suum with minimum loss of activity. By utilizing this method, 50 ml fraction was concentrated to a final volume of 3 ml in about 1.5 h without loss in enzyme activity. The concentrated enzyme had a specific activity of 64 units per mg. Ascaris muscle-cuticle was incubated in 50 1M solutions of either acetylcholine, serotonin, y-aminobutyric acid, levamisole, or saline alone. Phosphate analysis of the isolated phosphofructokinase from each incubation revealed that the enzyme contained the following moles of phosphate per subunit: 2.9 (acetylcholine), 2.2 (serotonin), ... continued below

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v, 39 leaves: ill.

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Kaeini, Mohammad R. (Mohammad Reza) August 1983.

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  • Kaeini, Mohammad R. (Mohammad Reza)

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A new procedure has been developed to concentrate the phosphofructokinase from muscle of Ascaris suum with minimum loss of activity. By utilizing this method, 50 ml fraction was concentrated to a final volume of 3 ml in about 1.5 h without loss in enzyme activity. The concentrated enzyme had a specific activity of 64 units per mg. Ascaris muscle-cuticle was incubated in 50 1M solutions of either acetylcholine, serotonin, y-aminobutyric acid, levamisole, or saline alone. Phosphate analysis of the isolated phosphofructokinase from each incubation revealed that the enzyme contained the following moles of phosphate per subunit: 2.9 (acetylcholine), 2.2 (serotonin), 2.0 (y-aminobutyric acid), 1.5 (levamisole), and 3.4 (salne alone). The present study did not establish a direct correlation between degree of phosphorylation and phosphofructokinase activity. Phosphofructokinase from muscle of Ascaris suum appears to contain several phosphorylation sites, and one of these sites is required to be phosphorylated in order for the enzyme to exhibit maximum activity under physiological conditions.

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v, 39 leaves: ill.

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  • August 1983

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  • May 10, 2015, 6:16 a.m.

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Kaeini, Mohammad R. (Mohammad Reza). Studies on the Purification and Phosphorylation of Phosphofructokinase from Ascaris suum, thesis, August 1983; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc503913/: accessed September 26, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .