Properties of Normal Rat Kidney Cells Transformed by a Temperature-Sensitive Mutant (LA31) of Rous Sarcoma Virus

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The basis of this investigation is to characterize growth property differences in normal versus virally transformed cells. Using a temperature-sensitive mutant of Rous sarcoma virus, the cells' transformation state is regulated by the growth temperature; at 33°C the cells are transformed, while at 39°C the cells have normal characteristics. The morphology of NRK cells is elongated and fibroblastic; when transformed the cells are rounded. Normal cells grow to a monolayer and stop, while transformed cells grow to saturation densities greater than just a monolayer amount. Transformed cells can form foci when grown in mixture with normal cells. Normal cells must ... continued below

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v, 39 leaves : ill.

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Connolly, John R. (John Robert) August 1983.

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  • Connolly, John R. (John Robert)

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The basis of this investigation is to characterize growth property differences in normal versus virally transformed cells. Using a temperature-sensitive mutant of Rous sarcoma virus, the cells' transformation state is regulated by the growth temperature; at 33°C the cells are transformed, while at 39°C the cells have normal characteristics. The morphology of NRK cells is elongated and fibroblastic; when transformed the cells are rounded. Normal cells grow to a monolayer and stop, while transformed cells grow to saturation densities greater than just a monolayer amount. Transformed cells can form foci when grown in mixture with normal cells. Normal cells must be in contact with the culture vessel in order to grow, but transformed cells lack anchorage dependence for growth.

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v, 39 leaves : ill.

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  • August 1983

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  • May 10, 2015, 6:16 a.m.

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  • Dec. 14, 2016, 9:52 a.m.

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Connolly, John R. (John Robert). Properties of Normal Rat Kidney Cells Transformed by a Temperature-Sensitive Mutant (LA31) of Rous Sarcoma Virus, thesis, August 1983; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc503838/: accessed October 16, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .