Regulation of an S6/H4 Kinase in Crude Lymphosarcoma P1798 Preparations

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Purified S6/H4 kinase (Mr 60,000) requires autophosphorylation for activation. A rabbit anti-S6/H4 kinase peptide (SVIDPVPAPVGDSHVDGAAK) antibody recognized both the S6/H4 kinase holoenzyme and catalytic domain. Immunoreactivity with p60 kinase protein, and S6/H4 kinase activity were precisely correlated in fractions obtained from ion exchange chromatography of P1798 lymphosarcoma extracts. An enzyme which catalyzed the MgATP-dependent phosphorylation and activation of S6/H4 kinase coeluted with immunoreactivity from Mono 5, but not Mono Q chromatography. Since S6/H4 kinase is homologous with rac-activated PAK65, the observation that phosphorylation is also required for activation suggests a complex mechanism for in vivo activation of the S6/H4 kinase.

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x, 67 leaves : ill.

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Taylor, Allison Antoinette December 1998.

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  • Taylor, Allison Antoinette

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Purified S6/H4 kinase (Mr 60,000) requires autophosphorylation for activation. A rabbit anti-S6/H4 kinase peptide (SVIDPVPAPVGDSHVDGAAK) antibody recognized both the S6/H4 kinase holoenzyme and catalytic domain. Immunoreactivity with p60 kinase protein, and S6/H4 kinase activity were precisely correlated in fractions obtained from ion exchange chromatography of P1798 lymphosarcoma extracts. An enzyme which catalyzed the MgATP-dependent phosphorylation and activation of S6/H4 kinase coeluted with immunoreactivity from Mono 5, but not Mono Q chromatography. Since S6/H4 kinase is homologous with rac-activated PAK65, the observation that phosphorylation is also required for activation suggests a complex mechanism for in vivo activation of the S6/H4 kinase.

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x, 67 leaves : ill.

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  • December 1998

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  • March 9, 2015, 8:15 a.m.

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  • Nov. 2, 2015, 9:50 a.m.

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Taylor, Allison Antoinette. Regulation of an S6/H4 Kinase in Crude Lymphosarcoma P1798 Preparations, thesis, December 1998; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc501281/: accessed October 22, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .