Internal Radiolabeling of Mycobacterial Antigens and Use in Macrophage Processing Studies

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Mycobacter avium complex serovars 4 and 20 were cultured in the presence of [3H] fucose, [3H]-methionine, and [3H]-mannose to specifically radiolabel the oligosaccharide of the glycopeptidolipid (GPL) antigens. Distribution of radioactivity in lipid was determined by thin-layer chromatographic methods. Examination of acid hydrolysates from radiolabeled antigens revealed that [3H]-methionine incorporated into methylated sugars in polar and apolar GPL components, whereas [3H]-mannose incorporated exclusively into the oligosaccharide of polar GPL antigens. Least incorporation of radiolabel into antigens was observed with [3H]-fucose. Use of radiolabeled serovar 4 antigens in macrophage uptake studies revealed maximum uptake to be slightly above 250 gg/ 3.2 ... continued below

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v, 70 leaves : ill.

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Woodbury, Julie L. (Julie Lynn) August 1988.

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  • Woodbury, Julie L. (Julie Lynn)

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Mycobacter avium complex serovars 4 and 20 were cultured in the presence of [3H] fucose, [3H]-methionine, and [3H]-mannose to specifically radiolabel the oligosaccharide of the glycopeptidolipid (GPL) antigens. Distribution of radioactivity in lipid was determined by thin-layer chromatographic methods. Examination of acid hydrolysates from radiolabeled antigens revealed that [3H]-methionine incorporated into methylated sugars in polar and apolar GPL components, whereas [3H]-mannose incorporated exclusively into the oligosaccharide of polar GPL antigens. Least incorporation of radiolabel into antigens was observed with [3H]-fucose. Use of radiolabeled serovar 4 antigens in macrophage uptake studies revealed maximum uptake to be slightly above 250 gg/ 3.2 x 105 cells. Timed experiments demonstrated that GPL antigens were relatively inert to degradation by resident peritoneal macrophages.

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v, 70 leaves : ill.

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  • Call Number: 379 N81
  • Accession or Local Control No: 1002779224-Woodbury
  • Archival Resource Key: ark:/67531/metadc500936

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UNT Theses and Dissertations

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  • August 1988

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  • March 9, 2015, 8:15 a.m.

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  • Dec. 4, 2017, 2:43 p.m.

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Citations, Rights, Re-Use

Woodbury, Julie L. (Julie Lynn). Internal Radiolabeling of Mycobacterial Antigens and Use in Macrophage Processing Studies, thesis, August 1988; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc500936/: accessed December 11, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .