Characterization of Pyrimidine Biosynthesis in Pseudomonas putida Using Mutant and Wild Type Strains

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The biosynthesis of pyrimidines in Pseudomonas putida was investigated. In this study, pyrimidine requiring mutants were isolated by conventional mutagenesis and enrichment. The strains required exogenously supplied pyrimidines for growth and were found by enzyme assays to be deficient for the product of the pyrB gene encoding the enzyme aspartate transcarbamoylase. None of the intermediates of the pathway could supply the auxotrophic requirement of the strain; only preformed pyrimidines, metabolized via salvage pathways could suffice. Pyrimidine limitation in the mutant caused a slight but significant fifty per cent increase in expression of all the de novo biosynthetic enzymes. Pyrimidine starvation's ... continued below

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vi, 68 leaves : ill.

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Chang, Mingren August 1991.

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  • Chang, Mingren

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The biosynthesis of pyrimidines in Pseudomonas putida was investigated. In this study, pyrimidine requiring mutants were isolated by conventional mutagenesis and enrichment. The strains required exogenously supplied pyrimidines for growth and were found by enzyme assays to be deficient for the product of the pyrB gene encoding the enzyme aspartate transcarbamoylase. None of the intermediates of the pathway could supply the auxotrophic requirement of the strain; only preformed pyrimidines, metabolized via salvage pathways could suffice. Pyrimidine limitation in the mutant caused a slight but significant fifty per cent increase in expression of all the de novo biosynthetic enzymes. Pyrimidine starvation's effect on nucleotide pool levels was examined in the mutant and caused a marked swelling of the purine nucleotide pools.

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vi, 68 leaves : ill.

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UNT Theses and Dissertations

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  • August 1991

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  • March 9, 2015, 8:15 a.m.

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  • Nov. 13, 2017, 8:32 a.m.

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Chang, Mingren. Characterization of Pyrimidine Biosynthesis in Pseudomonas putida Using Mutant and Wild Type Strains, thesis, August 1991; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc500647/: accessed November 18, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .