Characterization of the OCT Plasmid-Encoded Mercury Resistance Genetic Locus in Pseudomonas putida

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A 17.1 Kb genetic element encoding for mercury resistance (OCT-Hg^r) was shown to translocate from its original location on the OCT plasmid to the resistance plasmid, RPl, in Pseudomonas putida. Analysis of RPl-Hg^r recombinant plasmids revealed that insertion of mercury resistance genes into RPl could occur at a variety of sites, with all recombinants having common EcoRI restriction fragments of 9.4, 3.8, 2.3, and 1.6 Kb, derived from the insertion. Hybridization analysis suggested the existence of extensive homology between this insertion and the prototypic mercury resistance transposon, Tn501, as well as the location of a similar merA sequence. Although the ... continued below

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vi, 49 leaves: ill.

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Armbruster, Steven C. (Steven Christopher) May 1989.

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  • Armbruster, Steven C. (Steven Christopher)

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A 17.1 Kb genetic element encoding for mercury resistance (OCT-Hg^r) was shown to translocate from its original location on the OCT plasmid to the resistance plasmid, RPl, in Pseudomonas putida. Analysis of RPl-Hg^r recombinant plasmids revealed that insertion of mercury resistance genes into RPl could occur at a variety of sites, with all recombinants having common EcoRI restriction fragments of 9.4, 3.8, 2.3, and 1.6 Kb, derived from the insertion. Hybridization analysis suggested the existence of extensive homology between this insertion and the prototypic mercury resistance transposon, Tn501, as well as the location of a similar merA sequence. Although the overall size was shown to be quite different from Tn501, striking physical similarities are shared between these two elements.

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vi, 49 leaves: ill.

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UNT Theses and Dissertations

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  • May 1989

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  • March 9, 2015, 8:15 a.m.

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  • Nov. 27, 2017, 11:25 a.m.

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Citations, Rights, Re-Use

Armbruster, Steven C. (Steven Christopher). Characterization of the OCT Plasmid-Encoded Mercury Resistance Genetic Locus in Pseudomonas putida, thesis, May 1989; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc500381/: accessed February 25, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .