Analysis of cDNA libraries from developing seeds of guar (Cyamopsis tetragonoloba (L.) Taub)

PDF Version Also Available for Download.

Description

Article on the analysis of cDNA libraries from developing seeds of guar (Cyamopsis tetragonoloba (L.) Taub).

Physical Description

12 p.

Creation Information

Naoumkina, Marina A.; Torres-Jerez, Ivone; Allen, Stacy N.; He, Ji; Zhao, Patrick Xuechun; Dixon, R. A. et al. November 23, 2007.

Context

This article is part of the collection entitled: UNT Scholarly Works and was provided by UNT College of Arts and Sciences to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 115 times . More information about this article can be viewed below.

Who

People and organizations associated with either the creation of this article or its content.

Authors

Publisher

Provided By

UNT College of Arts and Sciences

The UNT College of Arts and Sciences educates students in traditional liberal arts, performing arts, sciences, professional, and technical academic programs. In addition to its departments, the college includes academic centers, institutes, programs, and offices providing diverse courses of study.

Contact Us

What

Descriptive information to help identify this article. Follow the links below to find similar items on the Digital Library.

Degree Information

Description

Article on the analysis of cDNA libraries from developing seeds of guar (Cyamopsis tetragonoloba (L.) Taub).

Physical Description

12 p.

Notes

Abstract: Background: Guar, Cyamopsis tetragonoloba (L.) Taub, is a member of the Leguminosae (Fabaceae) family and is economically the most important of the four species in the genus. The endosperm of guar seed is a rich source of mucilage or gum, which forms a viscous gel in cold water, and is used as an emulsifier, thickener and stabilizer in a wide range of foods and industrial applications. Guar gum is a galactomannan, consisting of a linear (1→4)-β-linked D-mannan backbone with single-unit, (1→6)-linked, α-D-galactopyranosyl side chains. To better understand regulation of guar seed development and galactomannan metabolism we created cDNA libraries and a resulting EST dataset from different developmental stages of guar seeds. Results: A database of 16,476 guar seed ESTs was constructed, with 8,163 and 8,313 ESTs derived from cDNA libraries I and II, respectively. Library I was constructed from seeds at an early developmental stage (15–25 days after flowering, DAF), and library II from seeds at 30–40 DAF. Quite different sets of genes were represented in these two libraries. Approximately 27% of the clones were not similar to known sequences, suggesting that these ESTs represent novel genes or may represent non-coding RNA. The high flux of energy into carbohydrate and storage protein synthesis in guar seeds was reflected by a high representation of genes annotated as involved in signal transduction, carbohydrate metabolism, chaperone and proteolytic processes, and translation and ribosome structure. Guar unigenes involved in galactomannan metabolism were identified. Among the seed storage proteins, the most abundant contig represented a conglutin accounting for 3.7% of the total ESTs from both libraries. Conclusion: The present EST collection and its annotation provide a resource for understanding guar seed biology and galactomannan metabolism.

Subjects

Source

  • BMC Plant Biology, 2007, London: BioMed Central Ltd.

Language

Item Type

Identifier

Unique identifying numbers for this article in the Digital Library or other systems.

Publication Information

  • Publication Title: BMC Plant Biology
  • Volume: 7
  • Issue: 62
  • Pages: 12
  • Peer Reviewed: Yes

Collections

This article is part of the following collection of related materials.

UNT Scholarly Works

Materials from the UNT community's research, creative, and scholarly activities and UNT's Open Access Repository. Access to some items in this collection may be restricted.

What responsibilities do I have when using this article?

When

Dates and time periods associated with this article.

Submitted Date

  • April 3, 2007

Accepted Date

  • November 23, 2007

Creation Date

  • November 23, 2007

Added to The UNT Digital Library

  • Jan. 22, 2015, 9:04 a.m.

Usage Statistics

When was this article last used?

Yesterday: 0
Past 30 days: 3
Total Uses: 115

Interact With This Article

Here are some suggestions for what to do next.

Start Reading

PDF Version Also Available for Download.

International Image Interoperability Framework

IIF Logo

We support the IIIF Presentation API

Naoumkina, Marina A.; Torres-Jerez, Ivone; Allen, Stacy N.; He, Ji; Zhao, Patrick Xuechun; Dixon, R. A. et al. Analysis of cDNA libraries from developing seeds of guar (Cyamopsis tetragonoloba (L.) Taub), article, November 23, 2007; [London, United Kingdom]. (digital.library.unt.edu/ark:/67531/metadc488178/: accessed June 20, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.