Regioselective synthesis of plant (iso)flavone glycosides in Escherichia coli

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Article on regioselective synthesis of plant (iso)flavone glycosides in Escherichia coli.

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8 p.

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He, Xian-Zhi; Li, Wen-Sheng; Blount, Jack W. & Dixon, R. A. August 2008.

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Article on regioselective synthesis of plant (iso)flavone glycosides in Escherichia coli.

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8 p.

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Abstract: The flavonoids genistein, biochanin A, luteolin, quercetin, and kaempferol are plant natural products with potentially useful pharmacological and nutraceutical activities. These natural products usually exist in plants as glycosides, and their glycosylation has a remarkable influence on their pharmacokinetic properties. The glycosyltransferases UGT71G1 and UGT73C8 from Medicago truncatula are excellent reagents for the regioselective glycosylation of (iso)flavonoids in Escherichia coli grown in Terrific broth. Ten to 20 mg/L of either genistein or biochanin A 7-O-glucoside was produced after feeding genistein or biochanin A to E. coli expressing UGT71G1, and similar levels of luteolin 4’-O- and 7-O-glucosides were produced after feeding luteolin to cultures expressing UGT73C8. For the production of kaempferol 3-O-glucoside or quercetin 3-O-glucoside, the Phe148Val or Tyr202Ala mutants of UGT71G1 were employed. Ten to 16 mg/L of either kaempferol 3-O- or quercetin 3-O-glucosides were produced on feeding kaempferol or quercetin to E. coli expressing these enzymes. More than 90% of the glucoside products were released to the medium, facilitating their isolation.

© The Author(s) 2008.

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  • Applied Microbiology and Biotechnology, 2008, New York: Springer Science+Business Media, pp. 253-260

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  • Publication Title: Applied Microbiology and Biotechnology
  • Volume: 80
  • Issue: 2
  • Page Start: 253
  • Page End: 260
  • Pages: 8
  • Peer Reviewed: Yes

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  • Supplementary materials: Regioselective synthesis of plant (iso)flavone glycosides in Escherichia coli, ark:/67531/metadc488167

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Supplementary materials: Regioselective synthesis of plant (iso)flavone glycosides in Escherichia coli (Text)

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Supplementary materials: Regioselective synthesis of plant (iso)flavone glycosides in Escherichia coli, ark:/67531/metadc488167

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  • August 2008

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  • Jan. 22, 2015, 9:04 a.m.

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  • Feb. 4, 2015, 3:19 p.m.

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He, Xian-Zhi; Li, Wen-Sheng; Blount, Jack W. & Dixon, R. A. Regioselective synthesis of plant (iso)flavone glycosides in Escherichia coli, article, August 2008; [New York, New York]. (digital.library.unt.edu/ark:/67531/metadc488164/: accessed August 21, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.