Metabolic engineering of proanthocyanidins through co-expression of anthocyanidin reductase and the PAP1 MYB transcription factor

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Article on the metabolic engineering of proanthocyanidins through co-expression of anthocyanidin reductase and the PAP1 MYB transcription factor.

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13 p. : col. ill.

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Xie, De-Yu; Sharma, Shashi B.; Wright, Elane; Wang, Z.-Y. (Zeng-Yu), 1963- & Dixon, R. A. February 13, 2006.

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Article on the metabolic engineering of proanthocyanidins through co-expression of anthocyanidin reductase and the PAP1 MYB transcription factor.

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13 p. : col. ill.

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Abstract: Proanthocyanidins (PAs) and their monomeric building blocks, the (epi)-flavan-3-ols, are plant antioxidants that confer multiple human health benefits. The presence of PAs in forage crops is an important agronomic trait, preventing pasture bloat in ruminant animals. However, many consumed plant materials lack PAs, and there has been little success to date in introducing monomeric or polymeric flavan-3-ols de novo into plant tissues for disease prevention by dietary means or development of ‘bloat-safe’ forages. We report the introduction of PAs into plants by combined expression of a MYB family transcription factor and anthocyanidin reductase for conversion of anthocyanidin into (epi)-flavan-3-ol. Tobacco leaves expressing both transgenes accumulated epicatechin and gallocatechin monomers, and a series of dimers and oligomers consisting primarily of epicatechin units. The levels of PAs reached values that would confer bloat reduction in forage species. Expression of anthocyanidin reductase in anthocyanin-containing leaves of the forage legume Medicago truncatula resulted in production of a specific subset of PA oligomers.

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© 2006 The Authors; Journal compilation © 2006 Blackwell Publishing Ltd., http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2006.02655.x/abstract

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  • The Plant Journal, 2006, Oxford: Blackwell Publishing Ltd., pp. 895-907

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  • Publication Title: The Plant Journal
  • Volume: 45
  • Issue: 6
  • Page Start: 895
  • Page End: 907
  • Pages: 13
  • Peer Reviewed: Yes

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  • February 13, 2006

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  • Jan. 22, 2015, 9:04 a.m.

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Xie, De-Yu; Sharma, Shashi B.; Wright, Elane; Wang, Z.-Y. (Zeng-Yu), 1963- & Dixon, R. A. Metabolic engineering of proanthocyanidins through co-expression of anthocyanidin reductase and the PAP1 MYB transcription factor, article, February 13, 2006; [Oxford, United Kingdom]. (digital.library.unt.edu/ark:/67531/metadc488145/: accessed June 20, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.