Engineering isoflavone metabolism with an artificial bifunctional enzyme

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Article on engineering isoflavone metabolism with an artificial bifunctional enzyme.

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12 p.

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Tian, Li & Dixon, R. A. February 16, 2006.

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  • Tian, Li Samuel Roberts Noble Foundation
  • Dixon, R. A. University of North Texas; Samuel Roberts Noble Foundation

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Article on engineering isoflavone metabolism with an artificial bifunctional enzyme.

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12 p.

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Abstract: Plant secondary metabolism has been a focus of research in recent years due to its significant roles in plant defense and in human medicine and nutrition. A protein engineering strategy was designed to more effectively manipulate plant secondary metabolite (isoflavonoid) biosynthesis. A bifunctional isoflavone synthase/chalcone isomerase (IFS/CHI) enzyme was constructed by in-frame gene fusion, and expressed in yeast and tobacco. The fusion protein was targeted to the endoplasmic reticulum (ER) membrane and the individual enzymatic functions of its component fragments were retained when assayed in yeast. Petals and young leaves of IFS/CHI transgenic tobacco plants produced higher levels of the isoflavone genistein and genistein glycosides as a ratio of total flavonoids produced than did plants transformed with IFS alone. Thus, through a combined molecular modeling, in vitro protein engineering and in planta metabolic engineering approach, it was possible to increase the potential for accumulation of isoflavonoid compounds in non-legume plants. Construction of bifunctional enzymes will simplify the transformation of plants with multiple pathway genes, and such enzymes may find broad uses for enzyme (e.g., cytochrome P450 family) and biochemical pathway engineering.

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  • Planta, 2006, Berlin: Springer-Verlag, pp. 496-507

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Publication Information

  • Publication Title: Planta
  • Volume: 224
  • Issue: 3
  • Page Start: 496
  • Page End: 507
  • Pages: 12
  • Peer Reviewed: Yes

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  • February 16, 2006

Added to The UNT Digital Library

  • Jan. 22, 2015, 9:04 a.m.

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Tian, Li & Dixon, R. A. Engineering isoflavone metabolism with an artificial bifunctional enzyme, article, February 16, 2006; [Berlin, Heidelberg]. (digital.library.unt.edu/ark:/67531/metadc488142/: accessed November 20, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.