A functional genomics approach to (iso)flavonoid glycosylation in the model legume Medicago truncatula

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Article on a functional genomics approach to (iso)flavonoid glycosylation in the model legume Medicago truncatula.

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20 p.

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Modolo, Luzia V.; Blount, Jack W.; Achnine, Lahoucine; Naoumkina, Marina A.; Wang, Xiaoqiang & Dixon, R. A. April 17, 2007.

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Article on a functional genomics approach to (iso)flavonoid glycosylation in the model legume Medicago truncatula.

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20 p.

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Abstract: Analysis of over 200,000 expressed sequence tags from a range of Medicago truncatula cDNA libraries resulted in the identification of over 150 different family 1 glycosyltransferase (UGT) genes. Of these, 63 were represented by full length clones in an EST library collection. Among these, 19 gave soluble proteins when expressed in E. coli, and these were screened for catalytic activity against a range of flavonoid and isoflavonoid substrates using a high-throughput HPLC assay method. Eight UGTs were identified with activity against isoflavones, flavones, flavonols or anthocyanidins, and several showed high catalytic specificity for more than one class of (iso)flavonoid substrate. All tested UGTs preferred UDP-glucose as sugar donor. Phylogenetic analysis indicated that the Medicago (iso)flavonoid glycosyltransferase gene sequences fell into a number of different clades, and several clustered with UGTs annotated as glycosylating non-flavonoid substrates. Quantitative RT-PCR and DNA microarray analysis revealed unique transcript expression patterns for each of the eight UGTs in Medicago organs and cell suspension cultures, and comparison of these patterns with known phytochemical profiles suggested in vivo functions for several of the enzymes.

© Springer Science+Business Media B.V. 2007. Kluwer Academic Publishers. http://link.springer.com/article/10.1007%2Fs11103-007-9167-6

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  • Plant Molecular Biology, 2007, New York: Springer Science+Business Media, pp. 499-518

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  • Publication Title: Plant Molecular Biology
  • Volume: 64
  • Issue: 5
  • Page Start: 499
  • Page End: 518
  • Pages: 20
  • Peer Reviewed: Yes

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UNT Scholarly Works

Materials from the UNT community's research, creative, and scholarly activities and UNT's Open Access Repository. Access to some items in this collection may be restricted.

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  • February 15, 2007

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  • March 21, 2007

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  • April 17, 2007

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  • Jan. 22, 2015, 9:04 a.m.

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Modolo, Luzia V.; Blount, Jack W.; Achnine, Lahoucine; Naoumkina, Marina A.; Wang, Xiaoqiang & Dixon, R. A. A functional genomics approach to (iso)flavonoid glycosylation in the model legume Medicago truncatula, article, April 17, 2007; [New York, New York]. (digital.library.unt.edu/ark:/67531/metadc488139/: accessed November 13, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.