Use of luminescence energy transfer probes to detect genetic variants.

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The purpose of this research was to study the hybridization of molecular beacons under different conditions and designs. Data collected suggest that the inconsistency found in the emission intensity of several of these probes may be caused by 3 important factors: length of the probe, nucleotide sequence and, the formation of an alternative complex structure such as a dimer. Of all three factors, dimer formation is the most troublesome, since it reduces the emission of the reporter molecules. A new probe design was used to reduce dimer formation. The emission signal of the improved probe was several folds stronger than ... continued below

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Vaccaro, Carlos August 2004.

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  • Vaccaro, Carlos

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Description

The purpose of this research was to study the hybridization of molecular beacons under different conditions and designs. Data collected suggest that the inconsistency found in the emission intensity of several of these probes may be caused by 3 important factors: length of the probe, nucleotide sequence and, the formation of an alternative complex structure such as a dimer. Of all three factors, dimer formation is the most troublesome, since it reduces the emission of the reporter molecules. A new probe design was used to reduce dimer formation. The emission signal of the improved probe was several folds stronger than those probes with the early design.
In this research, dimer formation is detected, furthermore a new probe with a different design was tested. If dimer formation can be reduced molecular beacons can be integrated into more complex hybridization systems providing an important tool in research and diagnosis of genetic disorders.

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  • August 2004

Added to The UNT Digital Library

  • Feb. 15, 2008, 3:34 p.m.

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  • Dec. 16, 2008, 9:52 a.m.

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Citations, Rights, Re-Use

Vaccaro, Carlos. Use of luminescence energy transfer probes to detect genetic variants., thesis, August 2004; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc4566/: accessed April 25, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .