Studies on actomyosin crossbridge flexibility using a new single molecule assay.

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Several key flexure sites exist in the muscle crossbridge including the actomyosin binding site which play important roles in the actomyosin crossbridge cycle. To distinguish between these sources of flexibility, a new single molecule assay was developed to observe the swiveling of rod about a single myosin. Myosins attached through a single crossbridge displayed mostly similar torsional characteristics compared to myosins attached through two crossbridges, which indicates that most of the torsional flexibility resides in the myosin subfragment-2, and thus the hinge between subfragment-2 and light meromyosin should contribute the most to this flexibility. The comparison of torsional characteristics in ... continued below

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Gundapaneni, Deepika May 2004.

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  • Gundapaneni, Deepika

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Several key flexure sites exist in the muscle crossbridge including the actomyosin binding site which play important roles in the actomyosin crossbridge cycle. To distinguish between these sources of flexibility, a new single molecule assay was developed to observe the swiveling of rod about a single myosin. Myosins attached through a single crossbridge displayed mostly similar torsional characteristics compared to myosins attached through two crossbridges, which indicates that most of the torsional flexibility resides in the myosin subfragment-2, and thus the hinge between subfragment-2 and light meromyosin should contribute the most to this flexibility. The comparison of torsional characteristics in the absence and presence of ADP demonstrated a small but significant increase in twist rates for the double-headed myosins but no increase for single-headed myosins, which indicates that the ADP-induced increase in flexibility arises due to changes in the myosin head and verifies that most flexibility resides in myosin subfragment-2.

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  • May 2004

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  • Feb. 15, 2008, 3:13 p.m.

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  • Dec. 15, 2008, 10:36 a.m.

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Gundapaneni, Deepika. Studies on actomyosin crossbridge flexibility using a new single molecule assay., thesis, May 2004; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc4514/: accessed December 13, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .