Factors affecting polyhydroxybutyrate accumulation in mesophyll cells of sugarcane and switchgrass

PDF Version Also Available for Download.

Description

Article on factors affecting polyhydroxybutyrate accumulation in mesophyll cells of sugarcane and switchgrass.

Physical Description

11 p.

Creation Information

McQualter, Richard B.; Somleva, Maria N.; Gebbie, Leigh K.; Li, Xuemei; Petrasovits, Lars A.; Snell, Kristi D. et al. September 10, 2014.

Context

This article is part of the collection entitled: UNT Scholarly Works and was provided by UNT College of Arts and Sciences to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 85 times . More information about this article can be viewed below.

Who

People and organizations associated with either the creation of this article or its content.

Authors

Publisher

Provided By

UNT College of Arts and Sciences

The UNT College of Arts and Sciences educates students in traditional liberal arts, performing arts, sciences, professional, and technical academic programs. In addition to its departments, the college includes academic centers, institutes, programs, and offices providing diverse courses of study.

Contact Us

What

Descriptive information to help identify this article. Follow the links below to find similar items on the Digital Library.

Degree Information

Description

Article on factors affecting polyhydroxybutyrate accumulation in mesophyll cells of sugarcane and switchgrass.

Physical Description

11 p.

Notes

Abstract: Background: Polyhydroxyalkanoates are linear biodegradable polyesters produced by bacteria as a carbon store and used to produce a range of bioplastics. Widespread polyhydroxyalkanoate production in C4 crops would decrease petroleum dependency by producing a renewable supply of biodegradable plastics along with residual biomass that could be converted into biofuels or energy. Increasing yields to commercial levels in biomass crops however remains a challenge. Previously, lower accumulation levels of the short side chain polyhydroxyalkanoate, polyhydroxybutyrate (PHB), were observed in the chloroplasts of mesophyll (M) cells compared to bundle sheath (BS) cells in transgenic maize (Zea mays), sugarcane (Saccharum sp.), and switchgrass (Panicum virgatum L.) leading to a significant decrease in the theoretical yield potential. Here we explore various factors which might affect polymer accumulation in mesophyll cells, including targeting of the PHB pathway enzymes to the mesophyll plastid and their access to substrate. Results: The small subunit of Rubisco from pea effectively targeted the PHB biosynthesis enzymes to both M and BS chloroplasts of sugarcane and switchgrass. PHB enzyme activity was retained following targeting to M plastids and was equivalent to that found in the BS plastids. Leaf total fatty acid content was not affected by PHB production. However, when fatty acid synthesis was chemically inhibited, polymer accumulated in M cells. Conclusions: In this study, we provide evidence that access to substrate and neither poor targeting nor insufficient activity of the PHB biosynthetic enzymes may be the limiting factor for polymer production in mesophyll chloroplasts of C4 plants.

Source

  • BMC Biotechnology, 2014, London: BioMed Central Ltd.

Language

Item Type

Identifier

Unique identifying numbers for this article in the Digital Library or other systems.

Publication Information

  • Publication Title: BMC Biotechnology
  • Volume: 14
  • Issue: 83
  • Pages: 11
  • Peer Reviewed: Yes

Collections

This article is part of the following collection of related materials.

UNT Scholarly Works

Materials from the UNT community's research, creative, and scholarly activities and UNT's Open Access Repository. Access to some items in this collection may be restricted.

What responsibilities do I have when using this article?

When

Dates and time periods associated with this article.

Submitted Date

  • June 9, 2014

Accepted Date

  • August 29, 2014

Creation Date

  • September 10, 2014

Added to The UNT Digital Library

  • Nov. 13, 2014, 7:28 a.m.

Usage Statistics

When was this article last used?

Yesterday: 0
Past 30 days: 3
Total Uses: 85

Interact With This Article

Here are some suggestions for what to do next.

Start Reading

PDF Version Also Available for Download.

Citations, Rights, Re-Use

McQualter, Richard B.; Somleva, Maria N.; Gebbie, Leigh K.; Li, Xuemei; Petrasovits, Lars A.; Snell, Kristi D. et al. Factors affecting polyhydroxybutyrate accumulation in mesophyll cells of sugarcane and switchgrass, article, September 10, 2014; [London, United Kingdom]. (digital.library.unt.edu/ark:/67531/metadc407877/: accessed January 22, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.