Hindrance of the Myosin Power Stroke Posed by the Proximity to the Troponin Complex Identified Using a Novel LRET Fluorescent Nanocircuit Page: 2
This thesis is part of the collection entitled: UNT Theses and Dissertations and was provided to UNT Digital Library by the UNT Libraries.
Extracted Text
The following text was automatically extracted from the image on this page using optical character recognition software:
Coffee Castro-Zena, Pilar G. Hindrance of the Myosin Power Stroke Posed by
the Proximity to the Troponin Complex Identified Usinq a Novel LRET Fluorescent
Nanocircuit. Master of Science (Biochemistry), May 2007, 49 pp., 1 table, 12
illustrations, references, 53 titles.
A novel luminescence resonance energy transfer (LRET) nanocircuit assay
involving a donor and two acceptors in tandem was developed to study the dynamic
interaction of skeletal muscle contraction proteins.
The donor transmits energy relayed to the acceptors distinguishing myosin
subfragment-1 (Sl) lever arm orientations. The last acceptor allows the detection of
Sl's bound near or in between troponin complexes on the thin filament. Additionally,
calcium related changes between troponin T and myosin were detected.
Based on this data, the troponin complex situated every 7 actin monomers,
hinders adjacently bound myosins to complete their power stroke; whereas myosins
bound in between troponin complexes undergo complete power strokes.
Upcoming Pages
Here’s what’s next.
Search Inside
This thesis can be searched. Note: Results may vary based on the legibility of text within the document.
Tools / Downloads
Get a copy of this page or view the extracted text.
Citing and Sharing
Basic information for referencing this web page. We also provide extended guidance on usage rights, references, copying or embedding.
Reference the current page of this Thesis.
Coffee Castro-Zena, Pilar G. Hindrance of the Myosin Power Stroke Posed by the Proximity to the Troponin Complex Identified Using a Novel LRET Fluorescent Nanocircuit, thesis, May 2007; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc3688/m1/2/: accessed April 25, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .