Mechanism of Activation by Autophosphorylation of an S6/H4 Kinase Isolated From Human Placenta Page: IV
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LIST OF ILLUSTRATIONS
Page
1. Flow diagram of the experimental strategy used to
determine the mechanism of S6/H4 kinase
activation by autophosphorylation............................................................... .25
2. Autoradiography of autophosphotylated p40................................................ 27
3. Two-dimensional map of p40 from in situ trypsin
digestion of p60............................................................................................ .29
4. HPLC analysis of trypsin digest pp40............................ 31
5. Effect of trypsin concentration and incubation
time on the phosphopeptides obtained from p40..................... 33
6. Effect of oxidation of thiol-containing amino acid
on the number of tryptic peptides obtained from autophosphorylated
p40.............................................35
7. Correlation of p40 autophosphorylation and activity
w ith activation tim e....................................................................................... 37
8. Quantification of tryptic phosphopeptides generated
from p40 activated with variable MgATP incubation
tim es........................................................................................................ . .... . . 39
9. Effect of exogenous substrate on autophosphorylation
and activation of p40 S61H4 kinase............................................................. .41
10. Autoradiography of autophosphorylated p60 and p60
phosphorylated in the presence of p40........................................................ .43
11. HPLC analysis of autophosphorylated p60 and p60
phosphorylated in the presence of p40........................... 45
12. Effect of p60 S6/H4 kinase phosphorylation on
phosphorylation sites and activation............................................................ . 47
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Benner, Gretchen E. (Gretchen Evonne). Mechanism of Activation by Autophosphorylation of an S6/H4 Kinase Isolated From Human Placenta, thesis, December 1994; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc332785/m1/4/: accessed April 19, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .