Photoactivatable Quantum Dots in Super-Resolution Microscopy of Muscle

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Super-resolution 3D imaging was achieved using newly synthesized photoactivatable quantum dot (PAQ dot) probes. Quantum dots were modified with a novel quencher system to make them photoactivatable. The unique properties of these PAQ dots enable single-fluorophore localization in three dimensions using a confocal microscopy optical sectioning method. Myosin and tropomyosin of rabbit myofibrilar bundles were specifically labeled with the newly synthesized PAQ dot. A sufficient number of single quantum dots were photoactivated, localized and reduced to their centroid and then reconstructed to a super-resolution image. The acquired super-resolution image shows a lateral and an axial sub-diffraction resolution and demonstrates ultrafine ... continued below

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viii, 72 p.: ill.

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Akel, Amal December 2010.

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This thesis is part of the collection entitled: UNT Theses and Dissertations and was provided by UNT Libraries to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 7562 times , with 86 in the last month . More information about this thesis can be viewed below.

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  • Akel, Amal

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Super-resolution 3D imaging was achieved using newly synthesized photoactivatable quantum dot (PAQ dot) probes. Quantum dots were modified with a novel quencher system to make them photoactivatable. The unique properties of these PAQ dots enable single-fluorophore localization in three dimensions using a confocal microscopy optical sectioning method. Myosin and tropomyosin of rabbit myofibrilar bundles were specifically labeled with the newly synthesized PAQ dot. A sufficient number of single quantum dots were photoactivated, localized and reduced to their centroid and then reconstructed to a super-resolution image. The acquired super-resolution image shows a lateral and an axial sub-diffraction resolution and demonstrates ultrafine striations with widths less than 70 nm that are not evident by conventional confocal microscopy. The striations appear to be related to nebulin thin filament binding protein. This newly developed imaging system is cutting edge for its high resolution and localization as well its simplicity and convenience.

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viii, 72 p.: ill.

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UNT Theses and Dissertations

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  • December 2010

Added to The UNT Digital Library

  • May 4, 2011, 1:11 p.m.

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  • Jan. 16, 2014, 4:19 p.m.

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Citations, Rights, Re-Use

Akel, Amal. Photoactivatable Quantum Dots in Super-Resolution Microscopy of Muscle, thesis, December 2010; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc33133/: accessed October 18, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .