Enhanced characteristics of genetically modified switchgrass (Panicum virgatum L.) for high biofuel production

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Article on enhanced characteristics of genetically modified switchgrass (Panicum virgatum L.) for high biofuel production.

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15 p. : col. ill.

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Shen, Hui; Poovaiah, Charleson R.; Ziebell, Angela; Tschaplinski, Timothy J.; Pattathil, Sivakumar; Gjersing, Erica et al. May 7, 2013.

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Article on enhanced characteristics of genetically modified switchgrass (Panicum virgatum L.) for high biofuel production.

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15 p. : col. ill.

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Abstract: Background: Lignocellulosic biomass is one of the most promising renewable and clean energy resources to reduce greenhouse gas emissions and dependence on fossil fuels. However, the resistance to accessibility of sugars embedded in plant cell walls (so-called recalcitrance) is a major barrier to economically viable cellulosic ethanol production. A recent report from the US National Academy of Sciences indicated that, “absent technological breakthroughs”, it was unlikely that the US would meet the congressionally mandated renewable fuel standard of 35 billion gallons of ethanol-equivalent biofuels plus 1 billion gallons of biodiesel by 2022. We here describe the properties of switchgrass (Panicum virgatum) biomass that has been genetically engineered to increase the cellulosic ethanol yield by more than 2-fold. Results: We have increased the cellulosic ethanol yield from switchgrass by 2.6-fold through overexpression of the transcription factor PvMYB4. This strategy reduces carbon deposition into lignin and phenolic fermentation inhibitors while maintaining the availability of potentially fermentable soluble sugars and pectic polysaccharides. Detailed biomass characterization analyses revealed that the levels and nature of phenolic acids embedded in the cell-wall, the lignin content and polymer size, lignin internal linkage levels, linkages between lignin and xylans/pectins, and levels of wall-bound fucose are all altered in PvMYB4-OX lines. Genetically engineered PvMYB4-OX switchgrass therefore provides a novel system for further understanding cell wall recalcitrance. Conclusions: Our results have demonstrated that overexpression of PvMYB4, a general transcriptional repressor of the phenylpropanoid/lignin biosynthesis pathway, can lead to very high yield ethanol production through dramatic reduction of recalcitrance. MYB4-OX switchgrass is an excellent model system for understanding recalcitrance, and provides new germplasm for developing switchgrass cultivars as biomass feedstocks for biofuel production.

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  • Biotechnology for Biofuels, 2013, London: BioMed Central

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  • Publication Title: Biotechnology for Biofuels
  • Volume: 6
  • Issue: 71
  • Pages: 15
  • Peer Reviewed: Yes

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UNT Scholarly Works

Materials from the UNT community's research, creative, and scholarly activities and UNT's Open Access Repository. Access to some items in this collection may be restricted.

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  • February 7, 2013

Accepted Date

  • April 30, 2013

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  • May 7, 2013

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  • Aug. 1, 2014, 8:31 a.m.

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Shen, Hui; Poovaiah, Charleson R.; Ziebell, Angela; Tschaplinski, Timothy J.; Pattathil, Sivakumar; Gjersing, Erica et al. Enhanced characteristics of genetically modified switchgrass (Panicum virgatum L.) for high biofuel production, article, May 7, 2013; [London, United Kingdom]. (digital.library.unt.edu/ark:/67531/metadc307519/: accessed April 23, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.