Multinetwork Nerve Cell Assay Platform with Parallel Recording Capability Page: 1
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(12) United States Patent
(54) MULTINETWORK NERVE CELL ASSAY
PLATFORM WITH PARALLEL RECORDING
Inventor: Guenter W. Gross, Denton, TX (US)
Assignee: University of North Texas, Denton, TX
( * ) Notice: Subject to any disclaimer, the term of this
patent is extended or adjusted under 35
U.S.C. 154(b) by 867 days.
Sep. 12, 2007
(2), (4) Date: Jul. 20, 2009
(87) PCT Pub. No.: W02008/100287
PCT Pub. Date: Aug. 21, 2008
Prior Publication Data
US 2009/0291466 Al Nov. 26, 2009
Related U.S. Application Data
(60) Provisional application No. 60/845,399, filed on Sep.
(51) Int. Cl.
C12M 1/34 (2006.01)
(52) U.S. Cl.
USPC .......... 435/287.1; 435/29; 435/285.2; 607/50
(58) Field of Classification Search
USPC ..... 435/29, 285.2, 287.1, 4, 7.2, 368; 607/50,
See application file for complete search history.
(10) Patent No.:
(45) Date of Patent:
US 8,460,921 B2
Jun. 11, 2013
U.S. PATENT DOCUMENTS
2002/0025573 Al * 2/2002 Maher et al. .......
200610173259 Al * 8/2006 Flaherty et al. ....
20060194255 Al * 8/2006 Finkel ................
... 435 7.1
Gramowski, Schiffmann and Gross, "Quantification of acute
neurotoxic effects of trimethyltin using neuronal networks cultured
on microelectrode arrays," NeuroToxicology, 21:331-42, 2000.
Gross and Gopal, Emerging histiotypic properties of cultured
neuronal networks. In: M. Taketani and M. Baudry (eds) Advances in
Network Electrophysiology using Multi-Electrode Arrays. Springer,
pp. 193-214. 2006.
Gross and Pancrazio Neuronal Network Biosensors. In: Smart
Biosensor Technology (G.K. Knopf and A.S. Bassi, eds), Taylor and
Francis Publishers, CRC Press.pp. 177-201, 2006.
Primary Examiner Michael Hobbs
(74) Attorney, Agent, or Firm - Jackson Walker L.L.P.
A neuronal network analysis plate having alternating rows of
recording wells and amplifying wells. The recording wells
contain a neural cell network and a series of electrodes for
recording the action potential signals of the neurons. The
electrodes are connected to amplifiers in adjacent amplifying
wells. The close proximity of these amplifiers ideal because it
permits the parallel, non-multiplexed recording of action
potential signals from multiple different active nerve cell
networks. The amplifiers in the amplifying wells can then be
connected to external amplification equipment. The neuronal
network analysis plate may be contained within a single com-
mercially available 24 or 96 well plate. The neuronal network
analysis plate can be used to detect and quantify pharmaco-
logical and toxicological responses of the neural cells to one
or more agents in vitro.
11 Claims, 3 Drawing Sheets
105 - .mm
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Gross, Guenter W. Multinetwork Nerve Cell Assay Platform with Parallel Recording Capability, patent, June 11, 2013; [Washington, D.C.]. (digital.library.unt.edu/ark:/67531/metadc306940/m1/1/: accessed February 21, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.