RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane Metadata

Metadata describes a digital item, providing (if known) such information as creator, publisher, contents, size, relationship to other resources, and more. Metadata may also contain "preservation" components that help us to maintain the integrity of digital files over time.

Available Formats

Dublin Core: XML Dublin Core: JSON Dublin Core: Text Dublin Core: RDF/XML UNTL: XML Access METS: XML

Title

  • Main Title RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane

Creator

  • Author: O'Neill, Brian P.
    Creator Type: Personal
    Creator Info: University of Queensland; BSES Limited
  • Author: Purnell, Matthew P.
    Creator Type: Personal
    Creator Info: University of Queensland; BSES Limited
  • Author: Nielsen, Lars K.
    Creator Type: Personal
    Creator Info: University of Queensland
  • Author: Brumbley, Stevens M.
    Creator Type: Personal
    Creator Info: University of Queensland; BSES Limited; University of North Texas

Publisher

  • Name: Springer Science+Business Media
    Place of Publication: [New York, New York]

Date

  • Creation: 2012-12-21

Language

  • English

Description

  • Content Description: Article discussing how RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane.
  • Physical Description: 6 p.

Subject

  • Keyword: RNA interference
  • Keyword: saccharum
  • Keyword: sucrose-derivatives
  • Keyword: sugarcane biofactory
  • Keyword: trehalase
  • Keyword: trehalase

Source

  • Journal: SpringerPlus, 2012, New York: Springer Science+Business Media

Citation

  • Publication Title: SpringerPlus
  • Volume: 1
  • Issue: 74
  • Pages: 6
  • Peer Reviewed: True

Collection

  • Name: UNT Scholarly Works
    Code: UNTSW

Institution

  • Name: UNT College of Arts and Sciences
    Code: UNTCAS

Rights

  • Rights Access: public
  • Rights License: by

Resource Type

  • Article

Format

  • Text

Identifier

  • DOI: 10.1186/2193-1801-1-74
  • Archival Resource Key: ark:/67531/metadc306718

Degree

  • Academic Department: Biological Sciences

Note

  • Display Note: Abstract: To engineer trehalose metabolism in sugarcane (Saccharum spp. hybrids) two transgenes were introduced to the genome: trehalose-6-phosphate synthase- phosphatase (TPSP), to increase trehalose biosynthesis and an RNAi transgene specific for trehalase, to abrogate trehalose catabolism. In RNAi-expressing lines trehalase expression was abrogated in many plants however no decrease in trehalase activity was observed. In TPSP lines trehalase activity was significantly higher. No events of co-integration of TPSP and RNAi transgenes were observed. We suggest trehalase activity is essential to mitigate embryonic lethal effects of trehalose metabolism and discuss the implications for engineering trehalose metabolism.
Back to Top of Screen