RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane

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Article discussing how RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane.

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6 p.

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O'Neill, Brian P.; Purnell, Matthew P.; Nielsen, Lars K. & Brumbley, Stevens M. December 21, 2012.

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Article discussing how RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane.

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6 p.

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Abstract: To engineer trehalose metabolism in sugarcane (Saccharum spp. hybrids) two transgenes were introduced to the genome: trehalose-6-phosphate synthase- phosphatase (TPSP), to increase trehalose biosynthesis and an RNAi transgene specific for trehalase, to abrogate trehalose catabolism. In RNAi-expressing lines trehalase expression was abrogated in many plants however no decrease in trehalase activity was observed. In TPSP lines trehalase activity was significantly higher. No events of co-integration of TPSP and RNAi transgenes were observed. We suggest trehalase activity is essential to mitigate embryonic lethal effects of trehalose metabolism and discuss the implications for engineering trehalose metabolism.

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  • SpringerPlus, 2012, New York: Springer Science+Business Media

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  • Publication Title: SpringerPlus
  • Volume: 1
  • Issue: 74
  • Pages: 6
  • Peer Reviewed: Yes

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  • December 21, 2012

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  • July 15, 2014, 5:33 a.m.

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O'Neill, Brian P.; Purnell, Matthew P.; Nielsen, Lars K. & Brumbley, Stevens M. RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane, article, December 21, 2012; [New York, New York]. (digital.library.unt.edu/ark:/67531/metadc306718/: accessed October 21, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.