Auditory Cortex Neurons: Primary Culture and Ion Channel Activity in Rat

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Article discussing auditory cortex neurons and primary culture and ion channel activity in rat.

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7 p.

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Moore, Ernest J.; Hall, Deanne B. & Narahashi, Toshio 1995.

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Article discussing auditory cortex neurons and primary culture and ion channel activity in rat.

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7 p.

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Abstract: We have developed a primary dissociated cell culture of the fetal (E17) and post-natal (P0-P10) rat auditory cortex. Pyramidal and non-pyramidal cells had a mean cross-sectional diameter of 12.73 ± 1.80 μm (mean ± S.D., n=25) and 17.58 ± 1.67 pm (mean ± S.D., n=10), respectively, measured at 6 days in culture. These cells were viable for as long as 18-21 days. They expressed voltage-gated sodium and potassium channel currents as early as one day in culture, and at various phases in cell culture. Sodium current, activated at membrane potentials more positive than -60 mV, displayed fast activation and inactivation kinetics. Fifty percent inactivation of sodium channels occurred at a pre-pulse potential of -63 mV. Delayed rectifier potassium channels were activated at potentials positive to -40 mV. Large hyperpolarizing constant current pulses elicited anode break action potentials, and large depolarizing constant current pulses exhibited rectification indicative of the delayed rectifying potassium channel activity.

Copyright © 1995 Scandinavian University Press.

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  • Acta Otolaryngol (Stockh), 1995, Oslo: Scandinavian University Press, pp. 776-782

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  • Publication Title: Acta Otolaryngol (Stockh)
  • Volume: 115
  • Issue: 2
  • Page Start: 776
  • Page End: 782
  • Peer Reviewed: Yes

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  • 1995

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  • June 6, 2014, 11:19 a.m.

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Moore, Ernest J.; Hall, Deanne B. & Narahashi, Toshio. Auditory Cortex Neurons: Primary Culture and Ion Channel Activity in Rat, article, 1995; [Oslo, Norway]. (digital.library.unt.edu/ark:/67531/metadc287992/: accessed October 23, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.