Virulence Factor Production in PyrE Mutants of Pseudomonas Aeruginosa

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It has been shown previously in our lab that mutations in the pyrimidine pathway reduced the ability of Pseudomonas aeruginosa to produce virulence factors. Knockout mutations in pyrB, pyrC and pyrD genes of the pyrimidine pathway showed that virulence factor production was decreased. Pyoverdin, pyocyanin, hemolysin, iron chelation, motility, and adherence are all considered virulence factors. Here I further investigate the effects of mutations in the pyrimidine pathway by studying a pyrE mutant. I studied the effect of the pyrE mutation on the production of the above virulence factors. Just like the effect of pyrB, pyrC and pyrD mutations,the pyrE ... continued below

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Niazy, Abdurahman May 2010.

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  • Niazy, Abdurahman

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It has been shown previously in our lab that mutations in the pyrimidine pathway reduced the ability of Pseudomonas aeruginosa to produce virulence factors. Knockout mutations in pyrB, pyrC and pyrD genes of the pyrimidine pathway showed that virulence factor production was decreased. Pyoverdin, pyocyanin, hemolysin, iron chelation, motility, and adherence are all considered virulence factors. Here I further investigate the effects of mutations in the pyrimidine pathway by studying a pyrE mutant. I studied the effect of the pyrE mutation on the production of the above virulence factors. Just like the effect of pyrB, pyrC and pyrD mutations,the pyrE mutation also showed that the bacteria were deficient in producing virulence factors when compared to the wild type. The broader impact of this research would be the possibility of finding drugs that could treat patients infected with P. aeruginosa and possibly extend the lives of chronically infected patients with cystic fibrosis.

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  • May 2010

Added to The UNT Digital Library

  • Sept. 10, 2010, 1:20 a.m.

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  • Jan. 12, 2011, 12:44 p.m.

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Niazy, Abdurahman. Virulence Factor Production in PyrE Mutants of Pseudomonas Aeruginosa, thesis, May 2010; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc28458/: accessed May 28, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .