Synthetic Peptides Model Instability of Cardiac Myosin Subfragment-2 Page: 3
This thesis is part of the collection entitled: UNT Theses and Dissertations and was provided to UNT Digital Library by the UNT Libraries.
Extracted Text
The following text was automatically extracted from the image on this page using optical character recognition software:
effect of HCM-causing-mutations, peptides containing E924K mutation and lethal mutation
E930 deletion were synthesized. Fluorescence resonance probes were conjugated to the
mutant peptides to determine coiled coil formation. Results obtained from both dynamic
simulations and resonance energy transfer experiments indicated that these mutations strongly
inhibit dimerization, and thus, destabilize coiled coil structure of the peptide. Further
experiments were conducted using heterodimers containing a chain of wildtype and a chain of
mutant peptide. Both E924K & Edel930 mutations destabilized coiled coil formation and
prevented dimerization. This peptide model system would provide a promising tool for drug
development targeting HCM-causing-mutations along the S2 region of myosin.
Upcoming Pages
Here’s what’s next.
Search Inside
This thesis can be searched. Note: Results may vary based on the legibility of text within the document.
Tools / Downloads
Get a copy of this page or view the extracted text.
Citing and Sharing
Basic information for referencing this web page. We also provide extended guidance on usage rights, references, copying or embedding.
Reference the current page of this Thesis.
Taei, Nasrin. Synthetic Peptides Model Instability of Cardiac Myosin Subfragment-2, thesis, August 2013; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc283844/m1/3/: accessed April 24, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .