A Genomics Approach to Deciphering Lignin Biosynthesis in Switchgrass

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Article on a genomics approach to deciphering lignin biosynthesis in switchgrass.

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21 p.

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Shen, Hui; Mazarei, Mitra; Hisano, Hiroshi; Escamilla-Treviño, Luis; Fu, Chunxiang; Pu, Yunqiao et al. November 2013.

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Article on a genomics approach to deciphering lignin biosynthesis in switchgrass.

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21 p.

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Abstract: It is necessary to overcome recalcitrance of the biomass to saccharification (sugar release) to make switchgrass (Panicum virgatum) economically viable as a feedstock for liquid biofuels. Lignin content correlates negatively with sugar release efficiency in switchgrass, but selecting the right gene candidates for engineering lignin biosynthesis in this tetraploid outcrossing species is not straightforward. To assist this endeavor, we have used an inducible switchgrass cell suspension system for studying lignin biosynthesis in response to exogenous brassinolide. By applying a combination of protein sequence phylogeny with whole-genome microarray analyses of induced cell cultures and developing stem internode sections, we have generated a list of candidate monolignol biosynthetic genes for switchgrass. Several genes that were strongly supported through our bioinformatics analysis as involved in lignin biosynthesis were confirmed by gene silencing studies, in which lignin levels were reduced as a result of targeting a single gene. However, candidate genes encoding enzymes involved in the early steps of the currently accepted monolignol biosynthesis pathway in dicots may have functionally redundant paralogues in switchgrass and therefore require further evaluation. This work provides a blueprint and resources for the systematic genome-wide study of the monolignol pathway in switchgrass, as well as other C4 monocot species.

Copyright © 2013 American Society of Plant Biologists. The following article appeared in The Plant Cell, 25:11, pp. 4342-4361, http://www.plantcell.org/content/25/11/4342.long

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  • The Plant Cell, 2013, Rockville: American Society of Plant Biologists, pp. 4342-4361

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  • Publication Title: The Plant Cell
  • Volume: 25
  • Issue: 11
  • Page Start: 4342
  • Page End: 4361
  • Peer Reviewed: Yes

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  • November 2013

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  • April 3, 2014, 8:46 p.m.

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  • Sept. 5, 2014, 11:12 a.m.

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Shen, Hui; Mazarei, Mitra; Hisano, Hiroshi; Escamilla-Treviño, Luis; Fu, Chunxiang; Pu, Yunqiao et al. A Genomics Approach to Deciphering Lignin Biosynthesis in Switchgrass, article, November 2013; [Rockville, Maryland]. (digital.library.unt.edu/ark:/67531/metadc282597/: accessed September 22, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.