MATE2 Mediates Vacuolar Sequestration of Flavonoid Glycosides and Glycoside Malonates in Medicago truncatula

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Article on MATE2 mediating vacuolar sequestration of flavonoid glycosides and glycoside malonates in Medicago truncatula.

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21 p.

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Zhao, Jian; Huhman, David; Shadle, Gail L.; He, Xian-Zhi; Sumner, Lloyd W.; Tang, Yuhong et al. April 2011.

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Article on MATE2 mediating vacuolar sequestration of flavonoid glycosides and glycoside malonates in Medicago truncatula.

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21 p.

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Abstract: The majority of flavonoids, such as anthocyanins, proanthocyanidins, and isoflavones, are stored in the central vacuole, but the molecular basis of flavonoid transport is still poorly understood. Here, we report the functional characterization of a multidrug and toxin extrusion transporter (MATE2), from Medicago truncatula. MATE 2 is expressed primarily in leaves and flowers. Despite its high similarity to the epicatechin 3′-O-glucoside transporter MATE1, MATE2 cannot efficiently transport proanthocyanidin precursors. In contrast, MATE2 shows higher transport capacity for anthocyanins and lower efficiency for other flavonoid glycosides. Three malonyltransferases that are coexpressed with MATE2 were identified. The malonylated flavonoid glucosides generated by these malonyltransferases are more efficiently taken up into MATE2-containing membrane vesicles than are the parent glycosides. Malonylation increases both the affinity and transport efficiency of flavonoid glucosides for uptake by MATE2. Genetic loss of MATE2 function leads to the disappearance of leaf anthocyanin pigmentation and pale flower color as a result of drastic decreases in the levels of various flavonoids. However, some flavonoid glycoside malonates accumulate to higher levels in MATE2 knockouts than in wild-type controls. Deletion of MATE2 increases seed proanthocyanidin biosynthesis, presumably via redirection of metabolic flux from anthocyanin storage.

Copyright © 2011 American Society of Plant Biologists. The following article appeared in The Plant Cell, 23:4, pp. 1536-1555, http://www.plantcell.org/content/23/4/1536.long

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  • The Plant Cell, 2011, Rockville: American Society of Plant Biologists, pp. 1536-1555

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  • Publication Title: The Plant Cell
  • Volume: 23
  • Issue: 4
  • Page Start: 1536
  • Page End: 1555
  • Peer Reviewed: Yes

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  • April 2011

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  • April 3, 2014, 8:46 p.m.

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  • Jan. 5, 2015, 12:54 p.m.

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Zhao, Jian; Huhman, David; Shadle, Gail L.; He, Xian-Zhi; Sumner, Lloyd W.; Tang, Yuhong et al. MATE2 Mediates Vacuolar Sequestration of Flavonoid Glycosides and Glycoside Malonates in Medicago truncatula, article, April 2011; [Rockville, Maryland]. (digital.library.unt.edu/ark:/67531/metadc282595/: accessed September 20, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.