Diversity of Bacteria at Healthy Human Conjunctiva

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Article on the diversity of bacteria at healthy human conjunctiva.

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6 p.

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Dong, Qunfeng; Brulc, Jennifer M.; Iovieno, Alfonso; Bates, Brandon; Garoutte, Aaron; Miller, Darlene et al. July 2011.

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Article on the diversity of bacteria at healthy human conjunctiva.

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6 p.

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Abstract: Purpose: Ocular surface (OS) microbiota contributes to infectious and autoimmune diseases of the eye. Comprehensive analysis of microbial diversity at the OS has been impossible because of the limitations of conventional cultivation techniques. This pilot study aimed to explore true diversity of human OS microbiota using DNA sequencing-based detection and identification of bacteria. Methods: Composition of the bacterial community was characterized using deep sequencing of the 16S rRNA gene amplicon libraries generated from total conjunctival swab DNA. The DNA sequences were classified and the diversity parameters measured using bioinformatics software ESPRIT and MOTHUR and tools available through the Ribosomal Database Project-II (RDP-II). Results: Deep sequencing of conjunctival rDNA from four subjects yielded a total of 115,003 quality DNA reads, corresponding to 221 species-level phylotypes per subject. The combined bacterial community classified into 5 phyla and 59 distinct genera. However, 31% of all DNA reads belonged to unclassified or novel bacteria. The intersubject variability of individual OS microbiomes was very significant. Regardless, 12 genera—Pseudomonas, Propionibacterium, Bradyrhizobium, Corynebacterium, Acinetobacter, Brevundimonas, Staphylococci, Aquabacterium, Sphingomonas, Streptococcus, Streptophyta, and Methylobacterium—were ubiquitous among the analyzed cohort and represented the putative “core” of conjunctival microbiota. The other 47 genera accounted for <4% of the classified portion of this microbiome. Unexpectedly, healthy conjunctiva contained many genera that are commonly identified as ocular surface pathogens. Conclusions: The first DNA sequencing-based survey of bacterial population at the conjunctiva have revealed an unexpectedly diverse microbial community. All analyzed samples contained ubiquitous (core) genera that included commensal, environmental, and opportunistic pathogenic bacteria.

Copyright © 2011 Association for Research in Vision and Ophthalmology. http://www.iovs.org/content/52/8/5408.long

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  • Investigative Ophthalmology and Visual Science, 2011, Rockville: Association for Research in Vision and Ophthalmology, pp. 5408-5413

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  • Publication Title: Investigative Ophthalmology and Visual Science
  • Volume: 52
  • Issue: 8
  • Page Start: 5408
  • Page End: 5413
  • Peer Reviewed: Yes

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  • July 2011

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  • April 3, 2014, 8:46 p.m.

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Dong, Qunfeng; Brulc, Jennifer M.; Iovieno, Alfonso; Bates, Brandon; Garoutte, Aaron; Miller, Darlene et al. Diversity of Bacteria at Healthy Human Conjunctiva, article, July 2011; [Rockville, Maryland]. (digital.library.unt.edu/ark:/67531/metadc282591/: accessed September 20, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.