Functional Characterization of Proanthocyanidin Pathway Enzymes from Tea and Their Application for Metabolic Engineering Metadata

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Title

  • Main Title Functional Characterization of Proanthocyanidin Pathway Enzymes from Tea and Their Application for Metabolic Engineering

Creator

  • Author: Pang, Yongzhen
    Creator Type: Personal
    Creator Info: Samuel Roberts Noble Foundation
  • Author: Abeysinghe, I. Sarath B.
    Creator Type: Personal
    Creator Info: Tea Research Institute of Sri Lanka
  • Author: He, Ji
    Creator Type: Personal
    Creator Info: Samuel Roberts Noble Foundation
  • Author: He, Xian-Zhi
    Creator Type: Personal
    Creator Info: Samuel Roberts Noble Foundation
  • Author: Huhman, David
    Creator Type: Personal
    Creator Info: Samuel Roberts Noble Foundation
  • Author: Mewan, K. Mudith
    Creator Type: Personal
    Creator Info: Tea Research Institute of Sri Lanka
  • Author: Sumner, Lloyd W.
    Creator Type: Personal
    Creator Info: Samuel Roberts Noble Foundation
  • Author: Yun, Jianfei
    Creator Type: Personal
    Creator Info: Samuel Roberts Noble Foundation
  • Author: Dixon, R. A.
    Creator Type: Personal
    Creator Info: University of North Texas; Samuel Roberts Noble Foundation

Publisher

  • Name: Association Society of Plant Biologists
    Place of Publication: [Rockville, Maryland]

Date

  • Creation: 2013-03

Language

  • English

Description

  • Content Description: Article on the functional characterization of proanthocyanidin pathway enzymes from tea and their application for metabolic engineering.
  • Physical Description: 14 p.

Subject

  • Keyword: tea
  • Keyword: Camellia sinensis
  • Keyword: metabolites
  • Keyword: polyphenolic proanthocyanidins

Source

  • Journal: Plant Physiology, 2013, Rockville: American Society of Plant Biologists, pp. 1103-1116

Citation

  • Publication Title: Plant Physiology
  • Volume: 161
  • Issue: 3
  • Page Start: 1103
  • Page End: 1116
  • Peer Reviewed: True

Collection

  • Name: UNT Scholarly Works
    Code: UNTSW

Institution

  • Name: UNT College of Arts and Sciences
    Code: UNTCAS

Rights

  • Rights Access: public

Resource Type

  • Article

Format

  • Text

Identifier

  • DOI: 10.1104/pp.112.212050
  • Archival Resource Key: ark:/67531/metadc282590

Degree

  • Academic Department: Biological Sciences

Note

  • Display Note: Abstract: Tea (Camellia sinensis) is rich in specialized metabolites, especially polyphenolic proanthocyanidins (PAs) and their precursors. To better understand the PA pathway in tea, we generated a complementary DNA library from leaf tissue of the blister blight-resistant tea cultivar TRI2043 and functionally characterized key enzymes responsible for the biosynthesis of PA precursors. Structural genes encoding enzymes involved in the general phenylpropanoid/flavonoid pathway and the PA-specific branch pathway were well represented in the library. Recombinant tea leucoanthocyanidin reductase (CsLAR) expressed in Escherichia coli was active with leucocyanidin as substrate to produce the 2R,3S-trans-flavan-ol (+)-catechin in vitro. Two genes encoding anthocyanidin reductase, CsANR1 and CsANR2, were also expressed in E. coli, and the recombinant proteins exhibited similar kinetic properties. Both converted cyanidin to a mixture of (+)-epicatechin and (−)-catechin, although in different proportions, indicating that both enzymes possess epimerase activity. These epimers were unexpected based on the belief that tea PAs are made from (−)-epicatechin and (+)-catechin. Ectopic expression of CsANR2 or CsLAR led to the accumulation of low levels of PA precursors and their conjugates in Medicago truncatula hairy roots and anthocyanin-overproducing tobacco (Nicotiana tabacum), but levels of oligomeric PAs were very low. Surprisingly, the expression of CsLAR in tobacco overproducing anthocyanin led to the accumulation of higher levels of epicatechin and its glucoside than of catechin, again highlighting the potential importance of epimerization in flavan-3-ol biosynthesis. These data provide a resource for understanding tea PA biosynthesis and tools for the bioengineering of flavanols.
  • Display Note: Copyright © 2013 American Society of Plant Biologists. The following article appeared in Plant Physiology, 161:3, pp. 1103-1116, http://www.plantphysiol.org/content/161/3/1103.long