Changes in Cell Wall Carbohydrate Extractability are Correlated with Reduced Recalcitrance of HCT Downregulated Alfalfa Biomass

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Article on changes in cell wall carbohydrate extractability and how these are correlated with reduced recalcitrance of hydroxycinnamoyl transferase (HCT) downregulated alfalfa biomass.

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5 p.

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Pattathil, Sivakumar; Saffold, Trina; Gallego-Giraldo, Lina; O'Neill, Malcolm; York, William S.; Dixon, R. A. et al. August 2012.

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This article is part of the collection entitled: UNT Scholarly Works and was provided by UNT College of Arts and Sciences to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 221 times . More information about this article can be viewed below.

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Article on changes in cell wall carbohydrate extractability and how these are correlated with reduced recalcitrance of hydroxycinnamoyl transferase (HCT) downregulated alfalfa biomass.

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5 p.

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Abstract: Downregulation of the expression of enzymes involved in lignin biosynthesis in alfalfa (Medicago sativa L.) has been shown to result in reduced cell wall recalcitrance and increased saccharification. Here we describe changes in the extractability of hemicellulosic and pectic polysaccharides in cell walls of alfalfa downregulated in the expression of hydroxycinnamoyl CoA: shikimate hydroxycinnamoyl transferase (HCT). Xylan and pectic polysaccharides are more readily solubilized from the cell walls of HCT downregulated transgenic lines than from empty vector control plants, suggesting alterations in lignin-polysaccharide interactions in the transgenic plants. Our studies show that reduced recalcitrance of HCT downregulated lines is also correlated with increased xylan extractability.

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  • Industrial Biotechnology, 2012, New Rochelle: Mary Ann Liebert, Inc.

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  • Publication Title: Industrial Biotechnology
  • Volume: 8
  • Issue: 4
  • Peer Reviewed: Yes

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UNT Scholarly Works

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  • August 2012

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  • March 31, 2014, 8:53 a.m.

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Pattathil, Sivakumar; Saffold, Trina; Gallego-Giraldo, Lina; O'Neill, Malcolm; York, William S.; Dixon, R. A. et al. Changes in Cell Wall Carbohydrate Extractability are Correlated with Reduced Recalcitrance of HCT Downregulated Alfalfa Biomass, article, August 2012; [New Rochelle, New York]. (digital.library.unt.edu/ark:/67531/metadc279713/: accessed September 19, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.