Spatial Mapping of Lipids at Cellular Resolution in Embryos of Cotton

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Article on the spatial mapping of lipids at cellular resolution in embryos of cotton.

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15 p.: ill.

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Horn, Patrick J.; Korte, Andrew R.; Neogi, Purnima B.; Love, Ebony; Fuchs, Johannes; Strupat, Kerstin et al. February 2012.

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Article on the spatial mapping of lipids at cellular resolution in embryos of cotton.

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15 p.: ill.

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Abstract: Advances in mass spectrometry (MS) have made comprehensive lipidomics analysis of complex tissues relatively commonplace. These compositional analyses, although able to resolve hundred of molecular species of lipids in single extracts, lose the original cellular context from which these lipids are derived. Recently, high-resolution MS Of individual lipid droplets from seed tissues indicated organelle-to-organelle variation in lipid composition, suggesting that heterogeneity of lipid distributions at the cellular level may be prevalent. Here, we employed matrix-assisted laser desorption/ionization-MS imaging (MALDI-MSI) approaches to visualize lipid species directly in seed tissues of upland cotton (Gossypium hirsutum). MS imaging of cryosections of mature cotton embryos revealed a distinct, heterogeneous distribution of molecular species of triacylglycerols and phosphatidylcholines, the major storage and membrane lipid classes in cotton embryos. Other lipids were imaged, including phosphatidylethanolamines, phosphatidic acids, sterols, and gossypols, indicating the broad range of metabolites and applications for this chemical visualization approach. We conclude that comprehensive lipidomics images generated by MALDI-MSI report accurate, relative amounts of lipid species in plant tissues and reveal previously unseen differences in spatial distributions providing for a new level of understanding in cellular biochemistry.

Copyright © 2012 American Society of Plant Biologists. The following article appeared in The Plant Cell 24:2, pp. 622-636, http://www.plantcell.org/content/24/2/622.long

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  • The Plant Cell, 2012, Rockville: American Society of Plant Biologists, pp. 622-636

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  • Publication Title: The Plant Cell
  • Volume: 24
  • Issue: 2
  • Page Start: 622
  • Page End: 636
  • Peer Reviewed: Yes

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UNT Scholarly Works

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  • February 2012

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  • March 31, 2014, 8:53 a.m.

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Horn, Patrick J.; Korte, Andrew R.; Neogi, Purnima B.; Love, Ebony; Fuchs, Johannes; Strupat, Kerstin et al. Spatial Mapping of Lipids at Cellular Resolution in Embryos of Cotton, article, February 2012; [Rockville, Maryland]. (digital.library.unt.edu/ark:/67531/metadc279702/: accessed November 19, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.