The Transcriptional Regulator AlgR Controls Cyanide Production in Pseudomonas aeruginosa

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Article on the transcriptional regulator AlgR controlling cyanide production in Pseudomonas aeruginosa.

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8 p.

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Carterson, Alexander J.; Morici, Lisa A.; Jackson, Debra W.; Frisk, Anders; Lizewski, Stephen E.; Jupiter, Ryan et al. October 2004.

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Article on the transcriptional regulator AlgR controlling cyanide production in Pseudomonas aeruginosa.

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8 p.

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Abstract: Pseudomonas aeruginosa is an opportunistic pathogen that causes chronic lung infections in cystic fibrosis (CF) patients. One characteristic of P. aeruginosa CF isolates is the overproduction of the exopolysaccharide alginate, controlled by AlgR. Transcriptional profiling analyses comparing mucoid P. aeruginosa strains to their isogenic algR deletion strains showed that the transcription of cyanide-synthesizing genes (hcnAB) was ~3-fold lower in the algR mutants. S1 nuclease protection assays corroborated these findings, indicating that AlgR activates hcnA transcription in mucoid P. aeruginosa. Quantification of hydrogen cyanide (HCN) production from laboratory isolates revealed that mucoid laboratory strains made sevenfold more HCN than their nonmucoid parental strains. In addition, comparison of laboratory and clinically derived nonmucoid strains revealed that HCN was fivefold higher in the nonmucoid CF isolates. Moreover, the average amount of cyanide produced by mucoid clinical isolates was 4.7 ± 0.85 μmol of HCN/mg of protein versus 2.4 ± 0.40 μmol of HCN/mg of protein for nonmucoid strains from a survey conducted with 41 P. aeruginosa CF isolates from 24 patients. Our data indicate that (i) mucoid P. aeruginosa regardless of their origin (laboratory or clinically derived) produce more cyanide than their nonmucoid counterparts, (ii) AlgR regulates HCN production in P. aeruginosa, and (iii) P. aeruginosa CF isolates are more hypercyanogenic than nonmucoid laboratory strains. Taken together, cyanide production may be a relevant virulence factor in CF lung disease, the production of which is regulated, in part, by AlgR.

Copyright © 2004, American Society for Microbiology. All Rights Reserved.

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  • Journal of Bacteriology, 2004, Washington DC: American Society for Microbiology, pp. 6837-6844

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  • Publication Title: Journal of Bacteriology
  • Volume: 186
  • Issue: 20
  • Page Start: 6837
  • Page End: 6844
  • Peer Reviewed: Yes

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  • October 2004

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  • March 31, 2014, 8:53 a.m.

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Carterson, Alexander J.; Morici, Lisa A.; Jackson, Debra W.; Frisk, Anders; Lizewski, Stephen E.; Jupiter, Ryan et al. The Transcriptional Regulator AlgR Controls Cyanide Production in Pseudomonas aeruginosa, article, October 2004; [Washington, DC]. (digital.library.unt.edu/ark:/67531/metadc279695/: accessed December 11, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.