Large P body-like RNPs form in C. elegans oocytes in response to arrested ovulation, heat shock, osmotic stress, and anoxia and regulated by the major sperm protein pathway

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Article on large P body-like RNPs forming in C. elegans oocytes in response to arrested ovulation, heat shock, osmotic stress, and anoxia and regulated by the major sperm protein pathway.

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34 p.: ill.

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Jud, Molly C.; Czerwinski, Michael J.; Wood, Megan P.; Young, Rachel A.; Gallo, Christopher M.; Bickel, Jeremy S. et al. June 1, 2008.

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Article on large P body-like RNPs forming in C. elegans oocytes in response to arrested ovulation, heat shock, osmotic stress, and anoxia and regulated by the major sperm protein pathway.

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34 p.: ill.

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Abstract: As C. elegans hermaphrodites age, sperm become depleted, ovulation arrests, and oocytes accumulate in the gonad arm. Large ribonucleoprotein (RNP) foci form in these arrested oocytes that contain RNA-binding proteins and translationally masked maternal mRNAs. Within 65 minutes of mating, the RNP foci dissociate and fertilization proceeds. The majority of arrested oocytes with foci result in viable embryos upon fertilization, suggesting that foci are not deleterious to oocyte function. We have determined that foci formation is not strictly a function of aging, and the somatic, ceh-18, branch of the major sperm protein pathway regulates the formation and dissociation of oocyte foci. Our hypothesis for the function of oocyte RNP foci is similar to the RNA-related functions of processing bodies (P bodies) and stress granules; here, we show three orthologs of P body proteins, DCP-2, CAR-1 and CGH-1, and two markers of stress granules, poly (A) binding protein (PABP) and TIA-1, appear to be present in the oocyte RNP foci. Our results are the first in vivo demonstration linking components of P bodies and stress granules in the germ line of a metazoan. Furthermore, our data demonstrate that formation of oocyte RNP foci is inducible in non-arrested oocytes by heat shock, osmotic stress, or anoxia, similar to the induction of stress granules in mammalian cells and P bodies in yeast. These data suggest commonalities between oocytes undergoing delayed fetilization and cells that are stressed environmentally, as to how they modulate mRNAs and regulate translation.

This is the accepted manuscript version of the article. Reprinted with permission from Elsevier Science Ltd., all rights reserved. The final definitive version is available here: http://dx.doi.org/10.1016/j.ydbio.2008.02.059

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  • Developmental Biology, 2008, New York: Elsevier Science Ltd.

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  • Publication Title: Developmental Biology
  • Volume: 318
  • Issue: 1
  • Page Start: 38
  • Page End: 51
  • Peer Reviewed: Yes

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  • June 1, 2008

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  • March 31, 2014, 8:53 a.m.

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  • April 1, 2015, 3:53 p.m.

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Jud, Molly C.; Czerwinski, Michael J.; Wood, Megan P.; Young, Rachel A.; Gallo, Christopher M.; Bickel, Jeremy S. et al. Large P body-like RNPs form in C. elegans oocytes in response to arrested ovulation, heat shock, osmotic stress, and anoxia and regulated by the major sperm protein pathway, article, June 1, 2008; [New York, New York]. (digital.library.unt.edu/ark:/67531/metadc279689/: accessed September 22, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.