Evaluation of the Chlorophyll/Fluorescence Sensor of the YSI Multiprobe: Comparison to an Acetone Extraction Procedure

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The purpose of this study was to examine the suitability of the YSI model 6600 Environmental Monitoring System (multiprobe) for long term deployment at a site in Lewisville Lake, Texas. Specifically, agreement between a laboratory extraction procedure and the multiprobe chlorophyll/fluorescence readings was examined. Preliminary studies involved determining the best method for disrupting algal cells prior to analysis and examining the precision and linearity of the acetone extraction procedure. Cell disruption by mortar and pestle grinding was preferable to bath sonication. Comparison of the chlorophyll/fluorescence readings from the multiprobe and the extraction procedure indicated that they were significantly correlated but ... continued below

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Lambert, Patricia May 2001.

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  • Lambert, Patricia

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The purpose of this study was to examine the suitability of the YSI model 6600 Environmental Monitoring System (multiprobe) for long term deployment at a site in Lewisville Lake, Texas. Specifically, agreement between a laboratory extraction procedure and the multiprobe chlorophyll/fluorescence readings was examined. Preliminary studies involved determining the best method for disrupting algal cells prior to analysis and examining the precision and linearity of the acetone extraction procedure. Cell disruption by mortar and pestle grinding was preferable to bath sonication. Comparison of the chlorophyll/fluorescence readings from the multiprobe and the extraction procedure indicated that they were significantly correlated but temperature dependent.

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  • May 2001

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  • Sept. 25, 2007, 9:26 p.m.

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  • Dec. 12, 2013, 2:29 p.m.

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Lambert, Patricia. Evaluation of the Chlorophyll/Fluorescence Sensor of the YSI Multiprobe: Comparison to an Acetone Extraction Procedure, thesis, May 2001; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc2794/: accessed September 20, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .