Fumarate Activation and Kinetic Solvent Isotope Effects as Probes of the NAD-Malic Enzyme Reaction Page: III
This dissertation is part of the collection entitled: UNT Theses and Dissertations and was provided to UNT Digital Library by the UNT Libraries.
Extracted Text
The following text was automatically extracted from the image on this page using optical character recognition software:
TABLE OF CONTENTS
Page
LIST OF TABLES....................................................................................vi
LIST OF ILLUSTRATIONS......................................................................vii
LIST OF ABBREVIATIONS......................................................................ix
PART I. MECHAMISM OF ACTIVATION OF THE NAD-MALIC
ENZYME FROM ASCARIS SUUM BY FUMARATE
CHAPTER
I. INTRODUCTION..........................................................................1
II. MATERIALS AND METHODS
Enzyme and chemicals............................................................9
Preparation of malate-2-D..................................................10
Substrate calibration..........................................................11
Metal chelate correction....................................................11
Kinetic studies......................................................................12
Isotope partitioning experiments....................................13
Data processing......................................................................16
III. THEORY OF ISOTOPE PARTITIONING........................................19
IV. RESULTS
Activation by fumarate........................................................23
Specificity of the activator site..................................24
Isotope partitioning from E:Mg:malate..........................24
Deuterium isotope effects..................................................25
in
Upcoming Pages
Here’s what’s next.
Search Inside
This dissertation can be searched. Note: Results may vary based on the legibility of text within the document.
Tools / Downloads
Get a copy of this page or view the extracted text.
Citing and Sharing
Basic information for referencing this web page. We also provide extended guidance on usage rights, references, copying or embedding.
Reference the current page of this Dissertation.
Lai, Chung-Jeng. Fumarate Activation and Kinetic Solvent Isotope Effects as Probes of the NAD-Malic Enzyme Reaction, dissertation, December 1992; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc278864/m1/4/: accessed April 19, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .