Nucleotide Sequence of a Bovine Arginine Transfer RNA Gene

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A single plaque-pure lambda clone designated λBA84 that hybridized to a ˆ32P-labeled bovine arginine tRNA was isolated from a bovine genomic library harbored in a lambda bacteriophage vector. A 2.3-kilobase segment of this clone was found to contain an arginine transfer RNAccg gene by Southern blot hybridization analysis and dideoxyribonucleotide DNA sequencing. This gene contains the characteristic RNA polymerase III split promoter sequence found in all eukaryotic tRNAs and a potential RNA polymerase III termination site, consisting of four consecutive thymine residues, in the 3'-flanking region. Several possible cis-acting promoter elements were found within the 5'-flanking region of the sequenced ... continued below

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vii, 52 leaves : ill.

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Eubanks, Aleida C. (Aleida Christine) May 1996.

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  • Eubanks, Aleida C. (Aleida Christine)

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A single plaque-pure lambda clone designated λBA84 that hybridized to a ˆ32P-labeled bovine arginine tRNA was isolated from a bovine genomic library harbored in a lambda bacteriophage vector. A 2.3-kilobase segment of this clone was found to contain an arginine transfer RNAccg gene by Southern blot hybridization analysis and dideoxyribonucleotide DNA sequencing. This gene contains the characteristic RNA polymerase III split promoter sequence found in all eukaryotic tRNAs and a potential RNA polymerase III termination site, consisting of four consecutive thymine residues, in the 3'-flanking region. Several possible cis-acting promoter elements were found within the 5'-flanking region of the sequenced gene. The function of these elements, if any, is unknown.

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vii, 52 leaves : ill.

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  • May 1996

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  • March 26, 2014, 9:30 a.m.

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  • July 22, 2014, 4:33 p.m.

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Eubanks, Aleida C. (Aleida Christine). Nucleotide Sequence of a Bovine Arginine Transfer RNA Gene, thesis, May 1996; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc278753/: accessed May 22, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .