Use of tRNA Gene Probes to Identify Polymorphic Loci in the Bovine Genome

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A 30-mer oligonucleotide probe encoding the "A box" and anticodon loop regions of a human glycine tRNA gene was used to isolate a 581bp DNA fragment from a bovine genomic DNA library. Although the cross-hybridizing segment of DNA was found not to encode any tRNA gene or pseudogene, a region with homology to the "C-element" of the "BOV-tA" type Alulike artiodactyl retroposons was identified. This cross-hybridization was determined to be the result of conserved RNA polymerase III promoter elements in the probe portion of the tRNA gene and these repetitive elements. A microsatellite repeat (TC) was also found associated with ... continued below

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vi, 183 leaves : ill.

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Shariat, Parvaneh August 1998.

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  • Shariat, Parvaneh

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A 30-mer oligonucleotide probe encoding the "A box" and anticodon loop regions of a human glycine tRNA gene was used to isolate a 581bp DNA fragment from a bovine genomic DNA library. Although the cross-hybridizing segment of DNA was found not to encode any tRNA gene or pseudogene, a region with homology to the "C-element" of the "BOV-tA" type Alulike artiodactyl retroposons was identified. This cross-hybridization was determined to be the result of conserved RNA polymerase III promoter elements in the probe portion of the tRNA gene and these repetitive elements. A microsatellite repeat (TC) was also found associated with this element. Future screening for bovine tRNA genes will require the use of a) longer probes and higher stringency hybridization conditions or b) the simultaneous screening with probes from the 5' and 3' ends of the gene which avoid the conserved Pol III promoter boxes.

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vi, 183 leaves : ill.

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  • August 1998

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  • March 24, 2014, 8:07 p.m.

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  • Sept. 23, 2014, 10:43 a.m.

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Citations, Rights, Re-Use

Shariat, Parvaneh. Use of tRNA Gene Probes to Identify Polymorphic Loci in the Bovine Genome, thesis, August 1998; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc278338/: accessed November 19, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .